产酶溶杆菌OH11双精氨酸转运系统关键基因tatC的功能分析  

Functional Analysis of Twin arginine Translocation Gene tatC in Lysobacter enzymogenes Strain OH11

作　　者：徐菲菲[1] 钱国良[1] 汪郁兰[1] 张娟[1] 范加勤[1] 胡白石[1] 刘凤权[1] 

机构地区：[1]南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,南京210095

出　　处：《中国生物防治学报》2013年第4期521-530,共10页Chinese Journal of Biological Control

基　　金：公益性行业(农业)科研专项(200903052;201003004;201203034);国家"863"计划项目(2011AA10A205);国家自然科学基金(31101478);教育部博士点基金(20100097120013);现代农业产业技术体系(nycytx-29-09);国家"948"项目(2010-C18)

摘　　要：本研究采用同源重组的方法对产酶溶杆菌Lysobacter enzymogenes OH11中双精氨酸转运系统关键基因tatC进行缺失突变,通过对突变体表型及表型相关基因表达分析来研究该基因在菌株OH11中的功能,重点分析该基因与产酶溶杆菌中热稳定抗真菌因子HSAF生物合成的关系。研究结果表明,基因tatC的突变显著提高了HSAF生物合成的产量,以及HSAF生物合成的关键基因pks/nrps转录水平;改变了菌株OH11的细胞形态,使细胞从棍棒状变为椭圆状;并降低了菌株OH11生物膜的形成和滑动能力以及在营养缺陷型(10%TSB)培养基中的生长速率,但不改变其在营养丰富培养基(100%TSB)中的生长速率。此外,与野生型OH11相比,基因tatC突变株不改变其蛋白酶、纤维素酶、β1,3葡聚糖酶和几丁质酶的产生水平以及其对瓜果腐霉Pythium apanidermatum、立枯丝核菌Rhizoctonia solani和禾谷镰刀菌Fusarium graminearum的拮抗能力。Twin-arginine translocation gene （tatC） of Lysobacter enzymogenes strain OHll was knocked out by double-cross homologue recombination. Functions of tatC were investigated by comparisons of phenotypes and phenotype-associated gene expressions between wild type and tatC mutant, more attentions were paid on the relationship between tatC and HSAF, an antifungal secondary metabolite produced by L. enzymogenes. Compared with wild-type OHll, mutation of tatC significantly increased the production of HSAF and the transcriptional expression level ofpks/nrps, which was a key gene responsible for HSAF biosynthesis. It was observed that the normal clavated cell morphology was changed into ellipticity when disruption of tatC in L. enzymogenes. Furthermore, mutation of tatC remarkably impacted the growth rate in nutrient-limiting broth, biofilm formation and gliding motility. However, MtatC mutation of tatC did not impact the growth rate in nutrient-rich broth, four-type lytic enzyme production, and inhibitory activities against Pythium apanidermatum, Rhizoctonia solani and Fusarium graminearum.

关 键 词：产酶溶杆菌 基因tatC 敲除 功能分析 

分 类 号：S476[农业科学—农业昆虫与害虫防治]