海南省穿刺巴斯德芽菌资源调查及其系统发育分析  

Resources Survey of Pasteuria penetrans and Their Phylogenetic Analysis in Hainan Province

作　　者：黄惠琴[1] 朱军[1] 商桑[1] 张晓黎[1] 张福特[1] 孙前光[1] 鲍时翔[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海口571101

出　　处：《中国生物防治学报》2013年第4期607-612,共6页Chinese Journal of Biological Control

基　　金："973"计划项目(2013CB127500);"973"计划项目前期研究专项(2010CB134506);"948"项目(2011-G25);国家自然科学基金项目(31170062);海南省科技项目(311080;KJHZ2013-11);海口市重点科技计划(2011-0099);中央级公益性科研院所基金(ITBB11-0302;ITBB11-0306)

摘　　要：穿刺巴斯德芽菌Pasteuriapenetrans是根结线虫的专性寄生菌，是最有潜力的根结线虫生防因子。为了解海南省穿刺巴斯德芽菌资源，本文首次对海南省根结线虫病区进行调查，发现穿刺巴斯德芽菌的分布与土壤类型、寄主植物、气温等因素相关。在获得的27个巴斯德芽菌16SrDNA克隆中，均与穿刺巴斯德芽菌具有较高同源性。其中，菌株wf2与穿刺巴斯德芽菌P．penetrans16SrDNA同源性为95．8％，在发育树上处于独立的分支，为巴斯德芽菌属的1个新种；菌株dal、da2、qh和th与P.penetrans16SrDNA同源性为97．5％~97．8％，在发育树上也处于独立的分支，很可能是巴斯德芽菌属的新种；另22株可归属为穿刺巴斯德芽菌。调查结果反映出海南省具有丰富的巴斯德芽菌资源。Pasteuria penetrans is an obligate parasite of root-knot nematodes and also described as the most potential biopesticide. In order to realize resources of P. penetrans, the survey of P. penetrans from root-knot nematode area in Hainan province was conducted. It was found that distribution ofP. penetrans was related to soil, host plants, temperature and other factors. Twenty-seven 16S rDNA clones ofPasteuria sp. were obtained, with strain wf2 most closely related to P. penetrans （95.8%）, strain dal, da2, qh and th closely related to P. penetrans （97.5%--97.8%）. The phylogenetic tree showed that they formed two separate branches that were distinct from P. penetrans and other related Pasteuria strains, so the five isolates probably represented novel species in the genus Pasteuria, and the other 22 isolates were identified as P. penetrans. It reflected that Pasteuria sp. had rich resources in Hainan province.

关 键 词：穿刺巴斯德芽菌 根结线虫 调查 16S RDNA 系统发育分析 

分 类 号：S476[农业科学—农业昆虫与害虫防治]