莫诺苷的大鼠组织分布  

Tissue distribution of morroniside in rats

作　　者：熊山[1,2] 李敬来[2] 吕圭源[1] 张振清[2] 

机构地区：[1]浙江中医药大学,杭州310053 [2]军事医学科学院毒物药物研究所,北京100850

出　　处：《军事医学》2013年第10期745-748,共4页Military Medical Sciences

基　　金：国家科技重大专项资助项目(2012ZX09301003-001-007)

摘　　要：目的研究莫诺苷在大鼠组织的分布特征。方法 24只雄性SD大鼠随机分为4组,单剂量(30 mg/kg)莫诺苷灌胃给药,分别于给药后5,45,120和360 min股动脉放血处死动物,解剖取组织。采用高效液相色谱-串联质谱联用法测定莫诺苷浓度。结果大鼠给药5 min即在小肠、胃、脾脏、肝脏和心脏达最高值,120 min在脂肪组织中达最高值,其余组织均在45 min达到最高值,360 min后各组织的药物浓度均降至较低水平。结论莫诺苷在大鼠体内的吸收和分布较快,药物入血后在体内各组织分布较为广泛,在组织中的消除亦较快,结果提示药物没有组织蓄积趋势。Objective To investigate the tissue distribution of morroniside in rats. Methods Twenty-four male SD rats were randomly divided into 4 groups. The samples of plasma and tissues were collected at 5,45, 120 and 360 min after oral administration of morroniside （30 mg/kg）. LC-MS/MS method was established to determine morroniside in the tissues of rats. Results Morroniside was orally administered in rats and the concentration of the drug reached the peak in the intestine ,stomach, spleen, liver and heart after 5 min and in fat after 120 min and in other tissues after 45 min, respectively. The concentration of morroniside in the tissues decreased to a very low level after 360 min. Conclusion Morroniside can be quickly absorbed, distributed and eliminated after oral administration in rats and no concentration accumulation is ohserved in the tissues.

关 键 词：莫诺苷 山茱萸 液相色谱-质谱联用 组织分布 

分 类 号：R285[医药卫生—中药学]