IL-1βmRNA在脑梗塞大鼠脑组织表达及针药结合治疗后的免疫干预机制  

Expression of IL- 1βmRNA in Rats of Cerebral Infarction and Immune Interventional Mechanism after the Combination of Medicine and Acupuncture

作　　者：颜培宇[1] 王亚贤[1] 于晓红[1] 代巧妹[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《针灸临床杂志》2013年第10期45-47,共3页Journal of Clinical Acupuncture and Moxibustion

基　　金：黑龙江省中医管理局项目;项目编号:ZHY08-W32

摘　　要：目的：观察脑梗塞大鼠IL—1βmRNA表达，探讨中药活脑康配合电针联合治疗方法对脑梗塞大鼠的免疫干预作用。方法：取健康Wistar大鼠（280～350g）120只，随机分为5组：正常对照组（A）、模型组（B）、中药活脑康组（C）、电针组（D）和中药活脑康配合电针联合治疗组（E），每组6只，各治疗组按治疗时间再分4组，分别为治疗2d、4d、6d和8d，每组6只。采用腔内线栓法复制脑梗塞模型，通过RT—PCR法观察各组实验大鼠脑组织IL-1βmRNA表达情况。结果：B组大鼠IL—113mRNA表达显著高于A组（P〈0．01）；治疗2天后，与B组比较，C组和E组大鼠IL一113mRNA表达明显下降，与D组和B组比较差异有显著性（P〈0．05）；治疗4d后，各治疗组大鼠IL-1βmRNA表达均明显下降，E组疗效显著，且维持到治疗6d和8d后，与c组、D组比较差异有显著性（P〈0．05）。结论：脑梗塞大鼠脑组织内IL-1βmRNA表达显著升高，电针在治疗早期效果显著，针药结合方法可在治疗中后期维持这种治疗作用，进而控制炎性损伤，促进脑组织修复；随着治疗时间的延长，这种优势更加明显。Objective ： To observe IL - 113 mRNA expression of the rats with cerebral infarction, explore the im- mune interventional effect by Chinese medicine and electro- acupuncture treatment. Methods：Healthy Wistar rats weighing 280 -350 g were randomly divided into five groups：normal control group （A group） , model group （B group） , Chinese medicine group （C group）, electro -acupuncture group （D group） and Chinese medicine and electro - acupuncture group （ E group）, each group with six rats. C - E groups according to the treatment time were divided into four groups secondly, respectively they were 2 d and 4 d, 6 d and 8d groups after the treatment. We prepared the rats of cerebral infarction by blocking on the right side of the brain artery of rats; observe the expression of IL - 113 mRNA of rats after treatment. Results： IL - 113 mRNA of group B was signifi- cantly higher than that in group A（ P 〈0.01 ） ;Compared with group B, IL- 115mRNA expressions of group C and E were declined obviously after treatment, and the difference was significant （ P 〈 0.05 ） ; After four days of treatment, IL - 113 mRNA expression of each treatment group was significantly decreased. Group E was signifi- cant, and maintain for the treatment lasted six days and eight days, compared with group C and D （ P 〈 0.05 ）. Conclusion： IL - 115 mRNA expression of group B significantly increases, and the effect of electro - acupuncture in the treatment of early stage is remarkable, and the combination of acupuncture with medicine can maintain the treatment effect in the late, then control inflammatory damage and promote brain tissue repairment, as the extension of treatment time, this advantage is more apparent.

关 键 词：IL-1ΒMRNA 脑梗塞 电针 中药 大鼠 

分 类 号：R246.6[医药卫生—针灸推拿学]