小鼠腰椎间盘退变与OPG基因的关系研究  

Lumbar Intervertebral Disc and Correlation Between OPG Gene Degeneration in Mice

作　　者：李恒善[1] 

机构地区：[1]呼和浩特市第一医院,内蒙古呼和浩特010030

出　　处：《内蒙古医学杂志》2013年第8期903-907,共5页Inner Mongolia Medical Journal

摘　　要：目的:比较骨保护素(OP G)基因敲除(OPG-/-)小鼠与正常(WT)小鼠腰椎间盘退变的关系。方法:分别取出生后4、8、12 W的OPG-/-小鼠及正常对照组小鼠的L4/5椎间盘,运用番红O-固绿染色法观察L4/5椎间盘形态学变化,测量椎间盘及软骨终板高度。应用免疫组化染色观察椎间盘聚集蛋白聚糖(aggrecan)基因表达量的变化。结果:1小鼠腰椎间盘番红O-固绿染色结果:OPG-/-小鼠的椎间盘软骨终板在第8W及第12 W时可观察到退化改变,软骨终板排列不规则,并有骨髓腔组织进入软骨终板及外层纤维环。o免疫组化染色结果显示:各年龄点OPG-/-组小鼠椎间盘aggrecan的蛋白表达均低于同龄WT组小鼠。OP G-/-小鼠软骨终板及椎间盘高度在4周时较对照组无明显变化,随着年龄的增长,第8周时较对照组降低,第12周时高度下降最明显(P<0.01)。结论:OPG基因缺失后可导致椎间盘退变,椎间盘正常的结构和功能的维持有赖于OPG基因。Objective： To observe the relationship between the degeneration of the lumbar intervertebral disc （IVD） in OPG gene knock - out mice and WT mice. Menthods： The L4/5 lumbar intervertebral discs （L4/5 IV-D） from 4 -, 8 -, 12 - week - old mice were harvested. Routine morphologic observations of the interverte- bral discs and cartilage endplate were performed using safranin - O/fast Green staining. The height of the inter- vertebral discs and the thickness of cartilage endplate were measured; Protein expressions of aggrecan in the discs were detected with immunohistochemical analysis. Results： Saffanin - O/fast green staining exhibited degenera- tion of the intervertebral discs and cartilage endplates in OPG-/- mice characterized with thinning, disordered arrangement of cartilage endplates and appearance of bone marrow cavity both in the endplate cartilage and annu- lus fibrosis. Immunohistochemical assay revealed significantly less protein expressions of aggrecan in the discs from OPG-/- mice than from wild type mice. Compared with the control group OPG-/- mice cartilage end- plate and intervertebral disc height have no significant change at 4W, Increase with age, lower than control group at 8W, height decreased significantly at 12 W（P 〈 0：01）. Conclusion： OPG gene plays a very important role in maintaining the structure and function of normal intervertebral disc, the deletion of OPG gene causes in- tervertebral disc degeration and vertebra osteo0orosis.

关 键 词：OPG 基因 椎间盘退变 

分 类 号：R681.53[医药卫生—骨科学]