多粘类芽孢杆菌培养条件优化研究  

Optimization of culture conditions of Paenibacillus polymyxa

作　　者：陈小煌[1] 李自然[1] 黄小云[1] 叶秀云[1] 刘波[2] 林娟[1] 

机构地区：[1]福州大学生物科学与工程学院,福建福州350116 [2]福建省农业科学院农业生物资源研究所,福建福州350003

出　　处：《福州大学学报（自然科学版）》2013年第5期947-954,共8页Journal of Fuzhou University(Natural Science Edition)

基　　金：973计划前期研究专项资助项目(2011CB111607);农业部公益性行业(农业)科研专项资助项目(201303094)

摘　　要：对多粘类芽孢杆菌菌株B-306的培养基配方及培养条件进行优化研究,优化后的多粘类芽孢杆菌B-306培养基配方及培养条件为:可溶性淀粉17.5 g·L-1、蛋白胨25.0 g·L-1、NaCl 1.25 g·L-1、Na2HPO40.5g·L-1、CaCl21.0 g·L-1,pH7.5;培养温度30℃、转速170 r·min-1、装液量80 mL/250 mL;接种量为10%(体积分数).经过以上条件优化,多粘类芽孢杆菌B-306的细胞生物量可达5.3×109cfu·mL-1,比优化前(3.17×108cfu·mL-1)提高了15.72倍.This study was performed to optimize the medium formula and culture conditions of Paenibacil- lus polymyxa B -306, which laid a foundation for the fermentation production and further development and utilization. The optimal medium formula for Paenibacillus polymyxa B - 306 consisted of 17.5 g·L^-1 solu- ble starch, 25.0 g·L^-1 peptone, 1.25 g ·L^-1 NaC1, O. 5 g ·L^-1 Na： HPO4 and 1.0 g·L^-1 CaC12, pH 7.5. And the optimal fermentation conditions were temperature of 30 %, rotation speed of 170 r·min^-1, liquid volume of 80 mL/250 mL and inoculation amount of 10% （V/V）. Under these conditions, the cell biomass of Paenibacillus polymyxa B -306 reached 5.3 ×10^9 cfu·niL^-1, which was increased by 15.72 times compared with those before the optimization （3.17×10^8 cfu · mL-1 ）.

关 键 词：多粘类芽孢杆菌 培养基配方 培养条件 优化 

分 类 号：Q939.9[生物学—微生物学]