铽-环丙沙星体系的荧光特性及环丙沙星的测定  

The Fluorescence Characteristic of Th^(3+) -Ciprefloxacin and the Determination of Ciprofloxacin

作　　者：吴淑清[1] 曹秋娥[2] 赵云昆[1] 张五卷[1] 陈兴国[1] 胡之德[1] 

机构地区：[1]兰州大学化学系,兰州730000 [2]云南大学化学系,昆明650091

出　　处：《分析化学》2000年第12期1462-1466,共5页Chinese Journal of Analytical Chemistry

摘　　要：近中性条件下，铽（Ⅲ）能与环丙沙星（ｃｉｐｒｏｆｌｏｘａｃｉｎ）反应形成１：２的络合物。利用这一反应，建立了简单、快速、灵敏的测定环丙沙星的荧光方法，并且详细讨论了测定的各种条件。测定的最佳条件为：λｗｘ／λｅｍ＝３２５．０／５４５．０ｎｍ，Ｔｂ３＋（３．０ ｘ １０－３ｍｏｌ／ Ｌ）２．０ ｍＬ，ｐＨ＝６．０的ＮａＡｃ－ＨＡｃ缓冲溶液 ２． ０ ｍＬ。线性范围 １３－ １０００ μｇ／Ｌ，回归方程为 Ｆ＝ ５． ４２＋ ０． ２２０７Ｃ（μｇ／Ｌ）（ｒ=０．９９８）；检出限为 １０μｇ／Ｌ。此方法用于血清及环丙沙星片剂的测定，结果令人满意。In near neutral medium, Terbium(III) can react with ciprofloxacin (CIP) to form 1: 2 coordination compound. Based on this reaction, we proposed a simple, rapid, sensitive new method for the fluorimetric determination of CIP and investigated the reaction conditions in detail. The optimum experimental conditions were as follows: the excitation and emission wavelengths of the complex were at 320.0 nm and 545.0 nm, respectively and 2.0 mL of 3.0 x 10-3 mol/L Tb3+, 2.0 mL of pH = 6.0 (NaAc(HAc) buffer solution were added. The linear range is 13-1000(μg/L). The regression equation is F = 5.42 + 0.2207C (μg/L) (r = 0.998) with the detection limit of 10μg/L. This method has been succeesfully applied to the determination of CIP in tablet and serum samples.

关 键 词：环丙沙星 铽(Ⅲ) 测定 荧光光度法 

分 类 号：TQ460[化学工程—制药化工]