稀土对大鼠代谢产物影响的核磁共振研究  

Effect of Rare Earth Element on the Metabolism in Rats by High Resolution Proton Nuclear Magnetic Resonance Spectroscopy

作　　者：李晓晶[1] 冯江华[1] 张善荣[1] 裴奉奎[1] 陈曦 聂毓秀[2] 

机构地区：[1]中国科学院长春应用化学研究所,长春130022 [2]白求恩医科大学,长春130021

出　　处：《分析化学》2000年第12期1472-1475,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金重大项目资助课题!(29890280)。

摘　　要：以高场核磁共振技术为研究手段，通过分析腹腔注射０．２、２、１０、２０ｍｇ／ｋｇ体重剂量 的Ｌａ（ＮＯ３）。后大鼠尿液中代谢物浓度、物种的变化，研究了稀土化合物在动物体内的作用 情况，结果表明稀土的引入使动物肾脏和肝脏都受到一定程度的损伤。并在代谢物中挑选出 了合适的ＮＭＲ　ｍａｒｋｅｒｓ，其变化可以反映稀土离子作用后大鼠的异常代谢。The high-field nuclear magnetic resonance (NMR) spectra can be used for the rapid multicomponent analysis in small amounts of biological fluids. In this paper, the effect of La(NO3 )3 on the rats metabolism in urine was investigated by 1H NMR analysis. The experimental groups of wistar rats were injected intraperitoneally with In(NO3)3 at doses of 0.2, 2.0, 10 and 20mg/kg body weight. The remarkable variation of low molecular weight metabolites in urine has been identified by 1H NMR spectra, in which dimethylamine, N, N-dimethylglycine, urea, a-ketoglutarate, trimethylamine N-oxide, succinate, citrate and amino acids have ho suggested as NMR markers for renal damage and ethanol, lactate, taurine as the markers for liver damage. This work may assess its possible use in the early detection of biochemical changes associated with Rare Earth induced kidney and liver dysfunction.

关 键 词：稀土 体液 核磁共振 代谢 药理 毒理 

分 类 号：O657.2[理学—分析化学]