流动注射化学发光测定盐酸土霉素  

Flow Injection- Chemiluminescence Determination of Oxytetracycline Hydrochloride

作　　者：聂峰[1] 王志银[1] 何云华[1] 

机构地区：[1]汉中师范学院化学系,汉中723000

出　　处：《分析化学》2000年第12期1516-1518,共3页Chinese Journal of Analytical Chemistry

基　　金：陕西省教委科研基金资助课题!(No.98JK018)。

摘　　要：水浴加热后的盐酸土霉素在磷酸介质中，过氧化氢存在下与高碘酸钠反应产生强烈的化学发光。结合流动注射技术建立了一种测定盐酸土霉素的化学发光新方法。方法的线性范围为１．０ｘ１０－８～６．０ｘ１０－６ｇ／ｍＬ盐酸土霉素。检出限为４．３ｘ１０－９ｇ／ｍＬ，ＲＳＤ为２．５％（Ｃｓ＝２．０ ｘ １０－６ｇ／ｍＬ；ｎ＝１１）。When oxytetracyline hydrochloride was heated in a boiling water bath, it reacted with sodium periodate and hydrogen peroxide in acidity medium, and then produced chemiluminescence signals. A new chemiluminescence method for determination of oxytetracycline hydrochloride using flow injection techniqe has been established.The linear range of the working curves was 1 .0 x 10-8 - 6 .0 x 10-6g/mL oxytetracycline hydrochloride.The detection limit of oxytetracycline hydrochloride was 4.3 x 10-9 g/mL. and relative standard deviation was 2.5% (Cs = 2.0 X 10-6 g/mL; n = 11).

关 键 词：化学发光 流动注射 盐酸土霉素 高碘酸钠 测定 

分 类 号：O657.3[理学—分析化学]