人血多形核白细胞呼吸爆发中活性氧与活性氧模拟酶的关系研究  

A Study on the Measurement of Active Oxygen During the Respiratory Burst in Polymorphonuclear Leucoyte and Relationship of Mimetic Enzyme as Potential Anti-active Oxygen

作　　者：冯清[1] 刘莉[1] 何永言[1] 王海龙[2] 李世新[3] 

机构地区：[1]同济医科大学化学教研室,武汉430030 [2]华中理工大学,武汉430070 [3]同济医科大学附属同济医院检验科,武汉430030

出　　处：《分析化学》2000年第11期1395-1397,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金!资助课题 (No .39370 794 )

摘　　要：用多形核白细胞 鲁米诺化学发光法测定具有超氧化物歧化酶 (SOD)和过氧化氢酶(CAT)双功能模拟酶 金属卟啉 (四磺酸基苯基铁卟啉、四磺酸基苯基铜卟啉、四磺酸基苯基锰卟啉、四磺酸基苯基钴卟啉 )对呼吸爆发所产生的活性氧的影响。结果表明 :模拟酶对爆发反应中细胞发光产生抑制 ,即对活性氧有清除作用 ;且同一物质随着浓度由 10 -7～ 10 -5mol L上升而增强。The active oxygen released by PMN during the respiratory burst was measured with PNM luminol enhanced chemiluminescence, the result showed that the metal porphyrins (tetraphenylporphyrinesulfonatoiron, tetraphenylporphyrinesulfonatocopper, tetraphenylporphyrinesulfonatomanganese, tetraphenylporphyrinesulfonatocobalt) used as dual function mimicry of superoxide dismutase (SOD) and catalase (CAT) inhibit chemiluminescence, scavenge the active oxygen. The percentage of scavenging active (O - 2, H 2O 2) increased with increasing concentration of the imitating compounds from 10 -7 mol/L to 10 -5 mol/L.

关 键 词：多形核白细胞 模拟酶 呼吸爆发 活性氧 化学发光 

分 类 号：Q554[生物学—生物化学]