亚甲基蓝共振光谱散射法测定脱氧核糖核酸  

Determination of Deoxyribonucleic Acid with Methylene Blue by a Resonance Light-Scattering Method

作　　者：向海艳[1] 陈小明[1] 李松青[1] 夏殊[1] 刘安喜[1] 

机构地区：[1]湘潭大学化学化工学院,湘潭411105

出　　处：《分析化学》2000年第11期1398-1340,共1页Chinese Journal of Analytical Chemistry

摘　　要：基于脱氧核糖核酸 (DNA)对有机染料亚甲基蓝的共振光散射的增强效应 ,拟定了一种测定DNA的共振光散射法。在pH5.5～ 7.5范围内 ,亚甲基蓝在 350nm处的共振光散射增强与DNA浓度呈线性关系 ,线性范围为 2 0 0～ 140 0 μg L ,检出限可达 15μg L。该方法简便、快速 ,用于合成样品中DNA的测定 ,结果令人满意。The resonance light scattering (RLS) of methylene blue (MB) is greatly enhanced by deoxyribonucleic acid (DNA) in the pH range of 5.5～7.5. The enhanced intensity of RLS at 350 nm is proportional to the concentration of DNA in the range of 0.2～1.4×10 -4 g/mL. The detection limit is 15×10 -9 g/mL. This method is simple, rapid, It has been applied to the determination of DNA in synthetic sample with satisfactory results.

关 键 词：亚甲基蓝 脱氧核糖核酸 共振光散射光 分析 

分 类 号：Q523.03[生物学—生物化学] O657.3[理学—分析化学]