荧光素钠荧光寿命的测定  

Determination of Fluorescence Lifetime of Sodium Fluorescein

作　　者：孙衍增[1] 

机构地区：[1]青岛大学医学院化学教研室,青岛266021

出　　处：《分析化学》2000年第11期1413-1416,共4页Chinese Journal of Analytical Chemistry

摘　　要：通过测定不同温度下荧光素钠溶液的荧光偏振度和粘度 ,根据Perrin公式测定荧光素钠的荧光寿命。在pH值为 8 5,溶液浓度为 1 6× 10 -5mol L的条件下 ,测得荧光素钠的荧光寿命为 3 4ns,P <0 .0 2 ,测定方法的准确度较高。The fluorescence polarity and viscosity of sodium fluorescein solution were measured under different temperatures. According to Perrin formula, the fluorescence lifetime was determined. Under the condition of pH=8.5, C =1.6×10 -5 mol/L, the fluorescence lifetime was 3.4ns, P <0.02. The proposed method has high accuracy.

关 键 词：荧光素钠 荧光寿命 荧光偏振度 测定 

分 类 号：R981.1[医药卫生—药品] R927[医药卫生—药学]