铜锌超氧化物歧化酶与精氨酸钴(Ⅱ)的相互作用  

Study on the interaction of copper-zinc superoxide dismutase with cobalt(Ⅱ)-arginine by spectral analysis

作　　者：郑学仿[1] 高大彬[1] 杨桦[2] 胡皆汉[3] 

机构地区：[1]大连大学化学化工系,辽宁大连116622 [2]大连理工大学生物工程系,辽宁大连116012 [3]中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《分子科学学报》2000年第4期230-233,共4页Journal of Molecular Science

基　　金：辽宁省科委自然科学基金资助项目! ( 972 0 67)

摘　　要：采用VIS ,ICP及酶活性测定等方法 ,研究铜锌超氧化物歧化酶(Cu2 Zn2 SOD)与精氨酸钴 (Ⅱ ) (Co(Arg) n)的直接相互作用以及外加精氨酸钴(Ⅱ )的量、溶液 pH值对此类相互作用的影响 .结果发现 :在水溶液中原酶活性中心处的Zn(Ⅱ )离子可被外加的精氨酸钴 (Ⅱ )部分诱导、交换出来 ,而溶液中外加Co(Arg) n 中的Co(Ⅱ )进入酶的活性中心 ,形成“Co—SOD”酶衍生物 ,并相应影响酶的催化活性 .与此同时 ,外加精氨酸钴 (Ⅱ )的量、溶液The effect of changing the amount of external added Co(Arg) n and pH values on the interaction of Cu 2Zn 2SOD with organic metal compound (Cobalt(Ⅱ)-Arginine) have been studied by means of ICP, VIS and measurement of enzyme activity. It has found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in the metalloenzyme (Cu 2Zn 2SOD) with external added Co(Arg) n . As a result, part of the metal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives (Co(Ⅱ)-substituted derivatives of SOD) were produced and the catalytic activity of enzyme were affected. It has also studied the intensity of interactions in different molar ratios (Cobalt(Ⅱ)-Arginine) and pH values and got some results under the effect of corresponding factors.

关 键 词：铜锌超氧歧化酶 精氨酸钴 相互作用 酶活测定 

分 类 号：Q554.9[生物学—生物化学]