Over-Expression of ScMnSOD,a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis  

作　　者：LIU Xiao-fei SUN Wei-min LI Ze-qin BAI Rui-xue LI Jing-xiao SHI Zi-han GENG Hong-wei ZHENG Ying ZHANG Jun ZHANG Gen-fa 

机构地区：[1]Beijing Key Laboratory of Gene Resource and Molecular Development,Beijing Normal University [2]College of Life Sciences,Beijing Normal University [3]COE For Neuroscience,Departments of Anesthesiology,Biomedical Sciences,Texas Tech University Health Sciences Center,Texas,79905,USA

出　　处：《Journal of Integrative Agriculture》2013年第10期1722-1730,共9页农业科学学报（英文版）

基　　金：supported by the National Natural Sciences Foundation of China (30970286, 31270362 and 31070289)

摘　　要：Jojoba（Simmondsia chinensis） is mainly distributed in desert,and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive.In this paper,we cloned and characterized a SOD gene from jojoba named as ScMnSOD,and introduced into Arabidopsis to investigate its functions of responding to drought stress.The transgenic Arabidopsis showed an improvement in drought tolerance.Moreover,under a water deficit condition,the accumulation of reactive oxygen species（ROS） was remarkably decreased in the transgenic lines compared to the WT.Furthermore,the ScMnSOD promoter was cloned to the 5′-upstream of GUS coding region in a binary vector,and introduced into Arabidopsis.And results showed that ScMnSOD expression can be induced by drought,salt,ABA,and low temperature.In conclusion,ScMnSOD plays an important role in drought tolerance which is,at least partially,attributed to its role in ROS detoxification.Jojoba（Simmondsia chinensis） is mainly distributed in desert,and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive.In this paper,we cloned and characterized a SOD gene from jojoba named as ScMnSOD,and introduced into Arabidopsis to investigate its functions of responding to drought stress.The transgenic Arabidopsis showed an improvement in drought tolerance.Moreover,under a water deficit condition,the accumulation of reactive oxygen species（ROS） was remarkably decreased in the transgenic lines compared to the WT.Furthermore,the ScMnSOD promoter was cloned to the 5′-upstream of GUS coding region in a binary vector,and introduced into Arabidopsis.And results showed that ScMnSOD expression can be induced by drought,salt,ABA,and low temperature.In conclusion,ScMnSOD plays an important role in drought tolerance which is,at least partially,attributed to its role in ROS detoxification.

关 键 词：drought jojoba promoter ROS ScMnSOD stress tolerance 

分 类 号：S332[农业科学—作物遗传育种]