The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Rasǒn Area of North Korea  

作　　者：JIA Li-jun ZHANG Shou-fa CAO Shi-nuo QIAN Nian-chao YU Long-zheng XUAN Xue-nan 

机构地区：[1]Laboratory of Veterinary Microbiology,Department of Veterinary Medicine,Yanbian University [2]National Research Center for Protozoan Diseases,Obihiro University of Agriculture and Veterinary Medicine,Inada-cho,Obihiro,Hokkaido,080-8555,Japan

出　　处：《Journal of Integrative Agriculture》2013年第10期1847-1854,共8页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (30560112, 30960278);Natural Science Foundation of Jilin Province of China (201115230)

摘　　要：The objective of this study was to investigate the epidemiology of bovine Piroplasms infections in the Rasǒn area of North Korea.The survey was carried out by light microscopic examination of Giemsa-stained blood smears,PCR,and phylogenetic evolution analysis of 128 blood samples collected from the Rasǒn area.The results showed that the infection rates of the small and large parasites were about 2.5 and 1.5% on average,respectively,in all Theileria sergenti and Babesia ovatapositive blood smears by microscopic examination of blood smears.The detection rate of T.sergenti Giemsa-stained smears was 43.75%,while that with PCR was 67.97%.The detection rate of B.ovata Giemsa-stained smears was 49.21%,while that with PCR was 71.88%.The sequence and phylogenetic analysis of DNA showed 98.84% homology between the 18S rRNA gene sequences of T.sergenti isolates from North Korean and that of Yanbian state from China,indicating the closest genetic relationship between both of them.Moreover,100% homology was shown between the 18S rRNA gene sequence of B.ovata isolates from North Korea and the published sequence AY081192 of GenBank,indicating the closest genetic relationship between both of them.This survey confirmed that Ras n is the endemic area of T.sergenti and B.ovata in North Korea.The objective of this study was to investigate the epidemiology of bovine Piroplasms infections in the Rasǒn area of North Korea.The survey was carried out by light microscopic examination of Giemsa-stained blood smears,PCR,and phylogenetic evolution analysis of 128 blood samples collected from the Rasǒn area.The results showed that the infection rates of the small and large parasites were about 2.5 and 1.5% on average,respectively,in all Theileria sergenti and Babesia ovatapositive blood smears by microscopic examination of blood smears.The detection rate of T.sergenti Giemsa-stained smears was 43.75%,while that with PCR was 67.97%.The detection rate of B.ovata Giemsa-stained smears was 49.21%,while that with PCR was 71.88%.The sequence and phylogenetic analysis of DNA showed 98.84% homology between the 18S rRNA gene sequences of T.sergenti isolates from North Korean and that of Yanbian state from China,indicating the closest genetic relationship between both of them.Moreover,100% homology was shown between the 18S rRNA gene sequence of B.ovata isolates from North Korea and the published sequence AY081192 of GenBank,indicating the closest genetic relationship between both of them.This survey confirmed that Ras n is the endemic area of T.sergenti and B.ovata in North Korea.

关 键 词：Rasǒn area of North Korea bovine Piroplasms phylogenetic trees 

分 类 号：S855.9[农业科学—临床兽医学]