羧苄西林钠对棉花愈伤组织诱导和生长的影响  

The Effect of Cerbenicillin Sodium on the Callus Induction and Growth in Cotton (Gossypium hirsutum)

作　　者：刘方[1] 张宝红[1] 

机构地区：[1]中国农业科学院棉花研究所农业部棉花遗传改良重点开放实验室,河南安阳455112

出　　处：《河南大学学报（自然科学版）》2000年第3期47-50,共4页Journal of Henan University:Natural Science

基　　金：农业部重点科研计划!"高新技术与基础研究"专题资助项目 (9710 49)

摘　　要：在一定范围内 ,羧苄西林钠对棉花愈伤组织的诱导和生长有促进作用 ,影响效果呈曲线分布 .对珂字 2 0 1的愈伤诱导表明 ,羧苄西林钠对棉花下胚轴的适宜浓度为 2 5 0～ 10 0 0mg/L ,对子叶的适宜浓度为 2 5 0～ 75 0mg/L .对珂字 2 0 1的愈伤组织增殖来说 ,羧苄西林钠初期抑制了棉花愈伤组织的生长 ,后期则促进生长 ,且随着羧苄西林钠浓度的增加 ,抑制时间也逐渐延长 .在 5 0 0mg/L的羧变西林钠浓度下添加 70mg/L的卡那霉素对棉花愈伤组织的诱导和生长有明显的致死作用 .Cerbencillin sodium promotes the formation and proliferation of callus from cotton (Gossypium hirsutum) in a certain concentration range. The effect was in a curve to the concentration. The optimum concentration is about 250～ 1?000 ?mg/L for the hypocotyl callus formation of Coker 201, and 250～750?mg/L for the cotyledon. cerbencillin sodium inhibited the proliferation of cotton callus of Coker 201 at beginning and promote in the later time. With the increase of cerbencillin sodium concentration, the inhibiting time was longer. The formation and proliferation of callus were inhibited and killed when cerbencillin sodium (concentration of 500?mg/L) and kannamycin(concentration of 70?mg/L) added at the same time.

关 键 词：羧苄西林钠 棉花 愈伤组织 遗传转化 农杆菌 

分 类 号：S562.035.3[农业科学—作物学] S336