多巴胺D1样受体在介导调节CD4^+T淋巴细胞功能中的作用  

Role of the dopamine D1-like receptor in mediating modulation of CD4^+ T cell function

作　　者：黄彦[1] 曹蓓蓓[1] 刘展[1] 邱一华[1] 

机构地区：[1]南通大学医学院生理学系,南通226001

出　　处：《南通大学学报（医学版）》2013年第5期339-342,共4页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(30870929);南通市应用研究计划(BK2012015);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：目的:从分子水平揭示CD4+T淋巴细胞表达D1受体和D5受体mRNA的现象,探讨这些受体在调节CD4+T淋巴细胞功能中的作用。方法:采用免疫磁珠分离和纯化小鼠淋巴结CD4+T淋巴细胞。用抗CD3/CD28抗体刺激活化CD4+T淋巴细胞,并用D1样受体激动剂SKF38393、拮抗剂SCH23390分别作用于CD4+T淋巴细胞,然后采用real-time PCR法检测CD4+T淋巴细胞上D1受体和D5受体mRNA的表达,Th1细胞特异性转录因子(T-box expressed in T cells,T-bet)和细胞因子干扰素γ(interferon-γ,IFN-γ)的表达,Th2细胞特异性转录因子GATA binding protein 3(GATA 3)和细胞因子白细胞介素-4(interleukin-4,IL-4)的表达,Th17细胞特异性转录因子(retinoid acid-related orphan receptor gammat,RoRγt)和细胞因子IL-17、IL-22的表达。结果 :D1样受体激动剂SKF38393(10-8和10-7mol/L)作用于CD4+T淋巴细胞后,CD4+T淋巴细胞表达D1样受体mRNA增高,IFN-γ的表达降低,GATA 3、IL-4的表达增高,但T-bet、RoRγt、IL-17和IL-22的表达没有变化,SCH23390(10-7mol/L)能阻断SKF38393的这些作用。结论:CD4+T淋巴细胞能表达多巴胺D1样受体,CD4+T淋巴细胞上D1样受体的激活可促进CD4+T淋巴细胞向Th2细胞分化、抑制Th1细胞功能及增强Th2细胞功能。Objective： To reveal the expression of D1 receptor and D5 receptor mRNA in CD4^＋ T lymphocytes, and to ex- plore their roles in mediating modulation of CD4^＋ T lymphocyte function. Methods： CD4^＋ T lymphocytes were purified from the mesenterie lymphnodes of mice by using CD4^＋ T-cell isolation kit. CD4^＋ T lymphocytes were incubated with anti-CD3/CD28 antibody and treated with selective D1-like receptor agonist（SKF38393） and antagonist（SCH23390） for 48 h. In the cultured CD4^＋ T lymphocytes with various drug exposures, the expression of two subtypes of D1-like receptors, Thl-specific tran- scription factor, T-bet, and cytokine interferon-γ（IFN-γ）, Th2-specific transcription factor, GATA 3, and cytokine IL-4, ThlT-specific transcription factor, RoR 7t, and eytokines, IL-17 and IL-22, were measured by real-time PCR. Results： The purified CD4^＋ T lymphocytes expressed D1-like receptor mRNA, including D1 and DS. After the cells were treated with D1- like receptor agonist SKF38393（10^-8 and 10^-7 mol/L）, the expression of D1 mRNAs was notably higher than that of SKF38393-untreated cells. Furthermore, SKF38393（10^-8 and 10^-7 mol/L） reduced the expression of IFN-γ, and increased the expression of IL-4 and GATA 3. SCH23390（10^-7 mol/L） Hocked these effects. But, the expression of T-bet, RoR γt, IL-17 and IL-22 that were treated with SKF38393 did not show remarkable change. Conclusions： CD4^＋ T lymphocytes can express D1-like receptors, including D1 receptor and D5 receptor. D1-like receptor in CD4^＋ T lymphoeytes mediated the inhibitory effects on function of Thl cells, and promoted that of Th2 cells.

关 键 词：D1样受体 CD4+T淋巴细胞 TH1细胞 TH2细胞 

分 类 号：R392.11[医药卫生—免疫学]