β-1,4-半乳糖基转移酶-Ⅰ在大鼠脑损伤后的表达分析  

The expression of β-1,4-galactosyltransferase-Ⅰ in brain injury

作　　者：陈建平[1] 李晓红[1] 杨曙梅[1] 杨春兰[1] 沈爱国[2] 程纯[2] 

机构地区：[1]南通大学附属医院检验科,南通226001 [2]南通大学医学院免疫学系

出　　处：《南通大学学报（医学版）》2013年第5期350-352,F0003,共4页Journal of Nantong University(Medical sciences)

基　　金：南通大学校级自然科学类科研基金项目(11Z012)

摘　　要：目的 :探讨脑损伤后β-1,4-半乳糖基转移酶-Ⅰ(β-1,4-galactosyltransferase-Ⅰ,β-1,4-GalT-Ⅰ)及其催化合成的β-1,4-半乳糖苷键(β-1,4-galactosylated carbohydrate,Galβ1-4GlcNAc)糖链与炎症的相关性。方法:运用免疫印迹法检测β-1,4-GalT-Ⅰ及Galβ1-4GlcNAc糖链在大鼠术后大脑皮质中的表达情况;采用免疫荧光双标,观察β-1,4-GalT-Ⅰ及Galβ1-4GlcNAc糖链在大脑皮质中的细胞定位。结果:大鼠脑损伤后,大脑皮质中β-1,4-GalT-Ⅰ及Galβ1-4GlcNAc糖链的表达水平呈现时间依赖关系,脑损伤后β-1,4-GalT-Ⅰ表达逐渐增加并持续在较高的水平,Galβ1-4GlcNAc糖链表达呈动态变化过程,在炎症早期上调达到高峰后有所降低。β-1,4-GalT-Ⅰ及其催化合成的Galβ1-4GlcNAc糖链主要定位于大脑皮质的活化的小胶质细胞中。结论:β-1,4-GalT-Ⅰ及其催化合成的Galβ1-4GlcNAc糖链在正常大脑皮质中仅有少量表达,脑损伤后两者表达均明显增高;术后高表达的β-1,4-GalT-Ⅰ主要定位于小胶质细胞,这提示该分子可能参与炎症介导的小胶质细胞的生物学行为的改变。Objective： To investigate the expression and locations changes of β-1,4-GalT- I and the total galactosylated of glycoproteins in rat models of brain injury. Methods： Western Blot and Lectin-blot were used to observe the expression of 15- 1,4-GalT-I and glycoproteins after brain injury. The localization of β-1,4-GalT- I and glycoproteins were investigated under fluorescence microscopy. Results： After surgery, the expression of β-1,4-GalT- I and galactosylated of glycoproteins changed in a time-dependent manner in the brain. The expression of β-1,4-GalT- I increased obviously and kept on the high level. Glyeoproteins were especially high at 3 d after brain injury, and then decreased gradually. Double labeling showed the presence of β-1,4-GalT- I and glycoproteins within actived microglia after brain injury. Conclusions： The results suggest that β-1,4-GalT- I and glycoproteins were upregulated after surgery. They localized mainly in actived microglia and participate in inflammatory microglia-mediated biological behavior changes.

关 键 词：脑损伤 β-1 4-半乳糖基转移酶-Ⅰ 小胶质细胞 大鼠 

分 类 号：R651.1[医药卫生—外科学]