丝组二肽所含基团在其切割DNA反应中的作用  

Seryl-histidine Dipeptide Side Chains Effects on Its DNA Cleavage Reaction

作　　者：万荣[1] 王宁[1] 赵刚 赵玉芬 

机构地区：[1]清华大学生命科学与工程研究院教育部生命有机膦化学开放实验室,北京100084

出　　处：《高等学校化学学报》2000年第12期1864-1866,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金! (批准号 :2 963 2 0 0 4)资助

摘　　要：The effects of the amino, hydroxyl, imidazole and carboxyl groups of Ser His on DNA cleavage reaction were studied. It was found that the amino and hydroxyl groups were essential for the DNA cleavage activity of Ser His, and that anyone of them blocked would result into the loss of cleavage activity. The carboxyl group was not the necessary group. The presence of the imidazole group would enhance the cleavage activity. However, the histidyl serine dipeptide, as the formula isomer of Ser His with the inverse peptide sequence, could not cleave DNA at all. Based on the above experimental results, a presumed cleavage mechanism of Ser His on DNA was proposed. [WT5HZ]The effects of the amino, hydroxyl, imidazole and carboxyl groups of Ser His on DNA cleavage reaction were studied. It was found that the amino and hydroxyl groups were essential for the DNA cleavage activity of Ser His, and that anyone of them blocked would result into the loss of cleavage activity. The carboxyl group was not the necessary group. The presence of the imidazole group would enhance the cleavage activity. However, the histidyl serine dipeptide, as the formula isomer of Ser His with the inverse peptide sequence, could not cleave DNA at all. Based on the above experimental results, a presumed cleavage mechanism of Ser His on DNA was proposed. [WT5HZ]

关 键 词：丝组二肽 DNA 切割 切割机理 人工核酸切割试剂 

分 类 号：Q523[生物学—生物化学]