共振喇曼光谱研究稀土离子Er^(3+)对肌红蛋白结构的影响  

Application of Resonance Raman Spectroscopy to Study the Effect of Rare-earth Ion Er^(3+) on Myoglobin

作　　者：赵雨[1] 徐金泽[2] 赵大庆 席时权 赵冰[2] 徐蔚清[2] 

机构地区：[1]稀土化学与物理开放实验室 [2]吉林大学超分子结构与谱学开放实验室,长春130023

出　　处：《高等学校化学学报》2000年第12期1913-1915,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 !(批准号 :2 963 3 0 10 )资助

摘　　要：The effect of rare earth ion Er 3+ on myoglobin(Mb) was studied by using Resonance Raman spectroscopy. The results show that with the variation of Er 3+ concentrations, both the oxidation state and spin state of Mb are sensitive to the perturbation of Er 3+ . Er 3+ added to Mb affects the oxidation and spin state synchronously. The structure sensitive groups of Mb are more accessible to the Er 3+ than other groups. According to the fluorometry and CD spectra studied and our results as mentioned above, we considered that Er 3+ does not interact with heme directly, and Er 3+ probably leads to the conformational changes of Mb due to the change of oxidation and spin state of Heme. [WT5HZ]The effect of rare earth ion Er 3+ on myoglobin(Mb) was studied by using Resonance Raman spectroscopy. The results show that with the variation of Er 3+ concentrations, both the oxidation state and spin state of Mb are sensitive to the perturbation of Er 3+ . Er 3+ added to Mb affects the oxidation and spin state synchronously. The structure sensitive groups of Mb are more accessible to the Er 3+ than other groups. According to the fluorometry and CD spectra studied and our results as mentioned above, we considered that Er 3+ does not interact with heme directly, and Er 3+ probably leads to the conformational changes of Mb due to the change of oxidation and spin state of Heme. [WT5HZ]

关 键 词：肌红蛋白 共振喇曼光谱 铒离子 结构 生物作用 

分 类 号：Q510.4[生物学—生物化学] Q581