他克莫司对小鼠髓样树突状细胞成熟及抗原呈递的干预作用研究  

Effects of Tacrolimus on Maturation and Antigen Presentation of Murine Bone Marrow-Derived Dendritic Cells

作　　者：周艳[1] 杨淑霞[2] 唐海明[2] 杨佩[2] 邹强[2] 王衍堂[2] 

机构地区：[1]四川大学华西第二医院急诊医学科,成都610041 [2]成都医学院基础医学院免疫教研室,成都610083

出　　处：《成都医学院学报》2013年第5期529-531,583,共4页Journal of Chengdu Medical College

基　　金：国家自然基金资助项目(NO:81202363);四川省教育厅资助项目(NO:13ZB0224);四川省卫生厅资助项目(NO:120257)

摘　　要：目的研究他克莫司(Tacrolimus,FK506)对小鼠骨髓来源的树突状细胞(bone marrow-derived dendritic cells,BM-DCs)成熟分化以及抗原呈递功能的影响。方法分离诱导imDCs、mDCs和FK-DCs,显微镜下观察细胞形态,流式细胞术检测表面分子CD80、CD86、CD40和MHC II的表达,ELISA检测培养上清中IL-12、IL-6及TNFα的分泌水平,CFSE细胞增殖试剂盒检测FK506对mDCs刺激同种异型的T细胞增殖的影响。结果与vehicle组相比,FK-DCs光镜下形态未见明显改变;mDCs CD80、CD86、CD40和MHC II表达均有明显下调,差异有统计学意义(P<0.05);细胞因子的分泌水平明显降低,差异有统计学意义(P<0.05);FK-DCs刺激同种异型T细胞增殖水平有明显降低(1∶10和1∶30组),差异有统计学意义(P<0.05)。结论 FK506在体外可显著抑制LPS刺激的DCs成熟和抗原呈递功能。Objective To study the effect of Tacrolimus on maturation and antigen presentation of murine bone marrow-derived dendritic cells. Methods The immature DCs （imDCs） ,mature DCs （mDCs） and FK DCs were isolated and cultured. The morphology was observed by light microscopy. The expression of CD80, CD86,CD40 and MHC II was detected by flow cytometry. EI.ISA was used to detect the expression of IL-12, IL 6 and TNFa. Proliferation of allotype T cell was tested by CFSE cell tracer kit in mixed lymphocyte reaction. Results Compared with vehicle, the morphology of FK-DCs was similar to imDCs, the expression of surface molecules and inflammatory cytokines were decreased significantly （P〈0.05） ;proliferation of allotype T cell stimulated by FK-DCs （1：10 and 1 ： 30 group） decreased significantly （P〈0.05）. Conclusion Taerolimus could significantly interfere with LPS- induced maturation and antigen presentation in dendritic cells in vitro.

关 键 词：他克莫司 树突状细胞 抗原呈递 

分 类 号：R392.5[医药卫生—免疫学]