拟南芥抗逆基因DREB2A转化大豆的研究  

Transformation of Soybean with Stress Related Gene DREB2A from Arabidopsis

作　　者：于志晶[1] 蔡勤安[1] 刘艳芝[1] 齐广勋[1] 马瑞[1] 

机构地区：[1]吉林省农业科学院农业生物技术研究所,吉林长春130033

出　　处：《大豆科学》2013年第5期606-608,共3页Soybean Science

基　　金：转基因生物新品种培育重大专项(2011ZX08004-002)

摘　　要：为提高大豆的耐旱耐盐碱性,将拟南芥转录因子DREB2A序列优化后,构建了植物表达载体pCAMBIA3300-DREB2A,利用农杆菌介导法将DREB2A基因导入大豆品种Willimas 82和Bert中,转化率分别为0.8%和3.2%。对抗性转基因植株进行PCR检测,2个品种分别获得阳性植株28和63株,表明DREB2A已整合到大豆基因组中。To improve the salt and drought tolerance of soybean,a vector of pCAMBIA3300-DREB2A with stress related gene DREB2A from Arabidopsis thaliana was constructed. DREB2A gene was introduced into soybean varieties Williams 82 and Bert by Agrobacterium-mediated transformation. The average transformation frequencies were 0.8% and 3.2% , respectively. Twenty-eight and 63 positive plants respectively were obtained by PCR identification. The results indicated that the gene DREB2A had been integrated into the genome of soybean.

关 键 词：大豆 DREB2A 耐盐耐旱 农杆菌介导遗传转化 

分 类 号：S565.1[农业科学—作物学]