电喷雾/飞行时间质谱法测定红霉素类抗生素的准确质量  

Determination of the Accurate Mass of Erythromycin Analogue Antibiotic by Electrospray Ionization/Time-of-flight Mass Spectrometry

作　　者：胡守刚[1] 郭寅龙[1] 吕龙[1] 

机构地区：[1]中国科学院上海有机化学研究所上,海200032

出　　处：《化学学报》2000年第11期1452-1456,共5页Acta Chimica Sinica

摘　　要：研究了聚乙二醇(PEG)为内标物的电喷雾(ESI)/飞行时间质谱(TOF-MS)准确质量测定方法,并应用于5个红霉素类抗生素质子化分子离子(MH^+)的质量测定.与理论值相比,相对误差均在5×10^(-6)以内.PEG可与K^+,Na^+,H^+等形成三种加合离子形式,通过选择适当的实验条件,控制PEG仅以其中的一种加合离子形式出现,这一特点扩大了其应用范围,使之可作为一种普适性的内标物.另外讨论了扫描质量范围、扫描速度等因素对测定结果的影响,并且比较了采用多峰校正法和两峰校正法的结果.结果表明,以PEG为内标的ESI/TOF质谱法可对不稳定碱性化合物的质子化分子离子(MH^+)进行准确质量测定,而且简便、快速.Using polyethylene glycol(PEG) as internal reference, electrospray ionization(ESI)/time - of -flight mass spectrometry(TOF - MS) was developed to determine the accurate mass of the protonated molecular ions of five erythromycin analogue antibiotics. Compared with the theoretical values, all relative errors were less than 5×10-6. PEG can form three adduct ions with K+ , Na+ , H+ , respectively, and may appear with single kind of adduct ions in suitable experiment conditions. PEG was recommended as a general internal reference compound. Besides, two - peak calibration is preferred to the multi - peak calibration. Scan mass width and scan speed were also studied, 104 - 144amu 5 seconds was recommended. Using polyethylene glycol(PEG) as internal reference, ESI/TOF mass spectrometry can determine the accurate mass of the protonated molecular ions of the unsteady basic compounds. This method is simple and expeditious.

关 键 词：电喷雾 飞行时间质谱 红霉素类抗生素 质量测定 

分 类 号：O657.63[理学—分析化学]