珍珠花丛生芽的诱导及生根条件的研究  

Studies on formation of the multiple-buds and rooting shoot tips of Staphylea bumalda DC

作　　者：刘幼琪[1] 洪艳艳[1] 陈永勤[1] 傅运生[1] 

机构地区：[1]湖北大学生命科学学院,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2000年第3期289-291,共3页Journal of Hubei University：Natural Science

基　　金：湖北省重点学科基金资助项目

摘　　要：诱导珍珠花 (StaphyleabumaldaDC)丛生芽的最佳培养基为MS基本培养基附加NAA 0 .2mg·L-1和BA 5mg·L-1,无菌苗在 1 /2MS培养基中附加NAA 1mg·L-1、活性碳 0 .4 5g·L-1、硫胺素 1mg·L-1、蔗糖 2 0g·L-1能长根形成完整植株 .Effects of some factors on formation of multiple-buds an d rooting shoot tips of Staphylea bumalda DC in vitro have been studied.The results showed that the kind and the concentration of hormone were the keys whi ch adjusted formation of multiple-buds.The highest rate of multiple-buds inducti on was formatted on MS medium containing BA 4～5 mg·L- -1 and N 0.2?mg· L- -1.Higher BA concentration would induce a great deal of callus forma tting.It was also found that the best rooting results were obtained in N 1?m g·L- -1. When the concentration of sucrose in medium was 15～20 g·L- -1the rooting rate could be marked risen. The experiment revealed that th e rooting rate was higher on 1/2 MS than on MS medium.The activated charcoal had remarkable effect on rooting.

关 键 词：珍珠花 丛生芽 生根 组织培养 快繁技术 

分 类 号：S793.9[农业科学—林木遗传育种] Q949.755.1[农业科学—林学]