基于UPLC-PDA法逍遥散乙醇提取物的石油醚萃取物血清药物化学初探  

Preliminary study on serum pharmacochemistry of petroleum ether fraction in ethanol extract from Xiaoyao Powder based on UPLC-PDA

作　　者：刘佳丽[1,2] 杨岚[1,2] 崔杰[1,2] 郭秉荣[1,2] 秦雪梅[1] 高晓霞[1] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006

出　　处：《中草药》2013年第20期2816-2822,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(81001688);国际科技合作项目(2011DFA32630);"重大新药创制"科技重大专项(2012ZX09103201-035);山西省基础研究青年科技研究基金(2011021007-1);山西省高等学校中青年拔尖创新人才支持计划资助项目

摘　　要：目的明确大鼠ig给予逍遥散乙醇提取物的石油醚萃取物(XYP-A)后血清中移行成分的归属与结构。方法建立大鼠ig给予XYP-A后血清中移行成分的UPLC-PDA色谱分析方法。对全方石油醚萃取物及柴胡(红柴胡)、当归、白术各单方石油醚萃取物分别ig给予大鼠后血清样品色谱图进行比较,分析血清中移行成分的归属,通过与对照品比对,比较保留时间、UV扫描图并结合文献数据对其定性。结果大鼠ig给予XYP-A后,血清中共检测到20个入血成分,其中12个为原形成分,分别来源于柴胡9个、当归2个、白术1个,另外8个为代谢产物,并指认了4个原形成分,分别为藁本内酯、白术内酯II、2,8,10-十五烷三烯-4,6-二炔-1-醇和柴胡炔醇。结论所建立的方法可行,血中检测到的20个移行成分很可能是逍遥散体内直接作用物质,为阐明该方药效物质基础提供了依据。Objective To study the adscription and identification of transitional constituents in serum of rats after ig administration of petroleum ether fraction in ethanol extract from Xiaoyao Powder （XYP-A）. Methods A UPLC-PDA method was established to identify the active components in serum of rats after ig administration of XYP-A and the single preparations of Bupleuri Radix （BR） Angelicae Sinensis Radix （ASR）, and Atractylodis Macrocephalae Rhizoma （AMR）. The transitional constituents were analyzed by comparing the fingerprints of the serum samples in formula, the single preparations and reference substances, referring literature, retention time, and UV scan spectra. Results Twenty components including 12 original components from XYP-A and eight metabolites were detected in serum of rats after ig administration of XYP-A. The original components consisted nine derived from BR, two from ASR, one from AMR, and the structures of ligustilide, atractylenolide II, 2, 8, 10-pentadecatriene-4, 6-diyne- 1-ol （CH- 1）, and bupleurynol （CH-2） were identified. Conclusion The method is successfully applied for the serum pharmaeochemistry study in rats. Twenty transitional constituents are absorbed into serum of rats and their metabolites may be the effective constituents of XYP-A acting directly to the body, which could lay the foundation for the serum pharmacodynamic study.

关 键 词：逍遥散 血清药物化学 移行成分 代谢物 UPLC—PDA 

分 类 号：R284.1[医药卫生—中药学]