辛芷鼻敏胶囊对变应性鼻炎大鼠血清中细胞因子和鼻黏膜组织中相关基因表达的影响  

Effects of Xinzhi Bimin Capsule on cytokines in serum and expression of related genes in nasal mucosa of rat model with allergic rhinitis

作　　者：杨清[1] 马冬均 姜孝芳[3] 王宏伟[3] 司红莉[3] 高丽[3] 

机构地区：[1]新疆医科大学第六附属医院,新疆乌鲁木齐830002 [2]乌鲁木齐市第一人民医院,新疆乌鲁木齐831400 [3]新疆医科大学,新疆乌鲁木齐830002

出　　处：《中草药》2013年第20期2889-2893,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30560144);新疆维吾尔自治区高校科研计划重点项目(XJEDU2006I041)

摘　　要：目的探讨辛芷鼻敏胶囊对变应性鼻炎大鼠的血清中白细胞介素-5(IL-5)、白细胞介素-13(IL-13)和鼻黏膜组织中的eotaxin、CCR3、信号转导和转录激活因子-6(STAT6)基因表达的影响,探讨其可能的作用机制。方法采用卵白蛋白制备变应性鼻炎大鼠模型。造模后大鼠随机分为模型组、辛芷鼻敏胶囊(2.3 g/kg)组、鼻炎康(1.8 g/kg)阳性对照组,每天给药1次,连续给药10 d,另设对照组。ELISA法检测各组大鼠血清IL-5、IL-13水平;用原位杂交(ISH)法半定量检测鼻黏膜中eotaxin、CCR3、STAT6基因的表达。结果辛芷鼻敏胶囊组大鼠血清IL-5、IL-13水平和鼻黏膜中STAT6基因表达明显低于模型组及鼻炎康组(P<0.05)。结论辛芷鼻敏胶囊可能通过下调STAT6基因表达,抑制大鼠变应性鼻炎发病过程中IL-5、IL-13的水平,达到减少嗜酸性粒细胞浸润进而改善变应性鼻炎症状的目的。Objective To explore the effects ofXinzhi Bimin Capsule （XBC） on the contents ofinterleukin （IL）-5 and IL-13 in serum and the expression of eotaxin, CCR3, and signal transducers and activators of transcription-6 （STAT6） in nasal mucosa of rat models with allergic rhinitis, and to identify the mechanism of pharmacodynamic action. Methods The allergic rhinitis rat model was established using ovalbumin, and forty rats were randomly divided into four groups, model, normal control, XBC （1.8 g/kg）, and Biyankang Tablet （1.8 g/kg, positive control） groups. The rats were administered once daily for 10 d. The contents of IL-5 and IL-6 were detected by ELISA, and the expression of positive cell of eotaxin, CCR3, and STAT6 in nasal mucosa was detected in semi quantity by in situ hybridization （ISH） method. Results The expression of STAT6 in nasal mucosa and the levels of IL-5 and IL-13 in blood serum were markedly lower in XBC group than those in the model and positive control groups （P 〈 0.05）. Conclusion XBC could reduce the expression of eotaxin, CCR3, and STAT6 in nasal mucosa of rats and down-regulate the contents of IL-5 and IL-13 in serum, so as to decrease the eosinophil infiltration and to treat allergic rhinitis in rats.

关 键 词：辛芷鼻敏胶囊 变应性鼻炎 细胞因子 基因表达 免疫调节 

分 类 号：R987[医药卫生—药品] R285.5[医药卫生—药学]