重离子辐照对绵羊精子的蛋白质组学影响研究  

Proteomics Analysis of Ram Sperm by Heavy Ion Radiation

作　　者：贺钰烜[1] 李鸿岩[2,3,4,5] 张红[2,3,4] 张勇[1] 贺建华 李润红[7] 赵兴绪[1] 张晶[1] 张小宇[1] 阚威[1] 李展[1] 王朝凤[1] 马艳粉[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国科学院近代物理研究所重离子辐射医学研究室,甘肃兰州730000 [3]中国科学院重离子束辐射生物医学重点实验室,甘肃兰州730000 [4]甘肃省重离子束辐射医学应用基础重点实验室,甘肃兰州730000 [5]中国科学院大学,北京100049 [6]甘肃省兰州市农业科学技术研究推广中心,甘肃兰州730030 [7]甘肃省定西师范高等专科学校生化系,甘肃定西744000

出　　处：《核农学报》2013年第10期1473-1478,共6页Journal of Nuclear Agricultural Sciences

基　　金：农业部转基因生物新品种培育重大专项(2008ZX08008-003);甘肃省重大科技专项(1102NKDA022);国家重点基础研究发展计划(973计划)(2010CB834202);国家自然科学基金(2008BADB2B04;2008BADB2B10-7);甘肃省农业生物技术研究与应用(GNSW-2008-15)

摘　　要：本研究为了分析由重离子辐照引起的绵羊精子蛋白质组学变化,用二维凝胶电泳分离精子蛋白,凝胶用考马斯亮蓝染色。PDQuest8.0软件检测分析差异表达蛋白斑点并经高效液相色谱串联离子阱质谱鉴定。结果显示:辐照后的精子凝胶图谱上有4个蛋白质斑点表达上调,1个蛋白质斑点缺失,这5个蛋白质斑点经鉴定后,在国际生物信息学中心数据库搜索到相对应的2种蛋白质:烯醇酶和烯醇酶1,在辐照后的精子中这2种蛋白质表达上调。研究表明:重离子辐照会引起绵羊精子蛋白质组学变化,通过对差异蛋白质的研究探索重离子辐照对绵羊精子生理功能的影响,为重离子辐照绵羊育种提供理论依据。The objective of this study was to investigate the proteome changes induced by heavy ion radiation using irradiated ram sperm by a two-dimensional electrophoresis （2 -DE） analysis. The 2D gels were stained with Coomassie Brilliant Blue. Differentially expressed proteins were detected by PDQuest 8.0 software and subjected to ion trap mass spectrometer equipped with a surveyor HPLC system, and differential protein spots were identified. Results showed there are five differential protein spots in irradiated sperm gels, four up-regulated protein spots and one spot missed. The differentially expressed protein spots were identified to be two up-regulated proteins including enolase, and enolase 1. It was concluded there was proteome changes induced by heavy ion radiation in ram sperm, which may be useful to clarify the physiology state of ram sperm in heavy ion radiation and provide a theoretical basis for radiation ram breeding.

关 键 词：绵羊 二维凝胶电泳分析 蛋白质组 重离子辐射 

分 类 号：S826[农业科学—畜牧学]