Inhibitory activity of an extract from a marine bacterium Halomonas sp.HSB07 against the red-tide microalga Gymnodinium sp.(Pyrrophyta)  

作　　者：刘娟 李富超 刘玲 姜鹏 刘兆普 

机构地区：[1]Key Laboratory of Marine Biology,Jiangsu Province,Nanjing Agricultural University [2]Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences [3]Nantong R&D Center of Marine Science and Technology,Institute of Oceanology,Chinese Academy of Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2013年第6期1241-1247,共7页中国海洋湖沼学报（英文版）

基　　金：Supported by the Science & Technology Project of Nantong(No.AS2011012);the National Key Technology Research and Development Program(No.2011BAE06B04-05);the Open Project Program of the Key Laboratory of Marine Bio-resources Sustainable Utilization,SCSIO,CAS(No.LMB121006)

摘　　要：In recent years,red tides occurred frequently in coastal areas worldwide.Various methods based on the use of clay,copper sulfate,and bacteria have been successful in controlling red tides to some extent.As a new defensive agent,marine microorganisms are important sources of compounds with potent inhibitory bioactivities against red-tide microalgae,such as Gymnodinium sp.(Pyrrophyta).In this study,we isolated a marine bacterium,HSB07,from seawater collected from Hongsha Bay,Sanya,South China Sea.Based on its 16S rRNA gene sequence and biochemical characteristics,the isolated strain HSB07 was identified as a member of the genus Halomonas.A crude ethyl acetate extract of strain HSB07 showed moderate inhibition activity against Gymnodinium sp.in a bioactive prescreening experiment.The extract was further separated into fractions A,B,and C by silica gel column chromatography.Fractions B and C showed strong inhibition activities against Gymnodinium.This is the first report of inhibitory activity of secondary metabolites of a Halomonas bacterium against a red-tide-causing microalga.In recent years, red tides occurred frequently in coastal areas worldwide. Various methods based on the use of clay, copper sulfate, and bacteria have been successful in controlling red tides to some extent. As a new defensive agent, marine microorganisms are important sources of compounds with potent inhibitory bioactivities against red-tide microalgae, such as Gymnodinium sp. （Pyrrophyta）. In this study, we isolated a marine bacterium, HSB07, from seawater collected from Hongsha Bay, Sanya, South China Sea. Based on its 16S rRNA gene sequence and biochemical characteristics, the isolated strain HSB07 was identified as a member of the genus Halomonas. A crude ethyl acetate extract of strain HSB07 showed moderate inhibition activity against Gymnodinium sp. in a bioactive prescreening experiment. The extract was further separated into fractions A, B, and C by silica gel column chromatography. Fractions B and C showed strong inhibition activities against Gymnodinium. This is the first report of inhibitory activity of secondary metabolites of a Halomonas bacterium against a red-tide-causing microalga.

关 键 词：red tides marine bacteria GYMNODINIUM HALOMONAS inhibition activity 

分 类 号：X55[环境科学与工程—环境工程]