Aromatic Moieties from Matured Chicken Manure and Agriculture Residues Compost Suppress Growth of Lepidium sativum L. and Trichoderma harzianum  

作　　者：LUO Yi-Hong ZHANG Dong-Qing YU Guang-Hui SHEN Qi-Rong 

机构地区：[1]Agricultural Ministry Key Lab of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River,Jiangsu Key Lab for Organic Solid Waste Utilization,College of Resources and Environmental Sciences,Nanjing Agricultural University [2]Baoshan Environmental Protection Bureau

出　　处：《Pedosphere》2013年第6期826-834,共9页土壤圈（英文版）

基　　金：Supported by the National Basic Research Program(973 Program)of China(No.2011CB100503);the National Natural Science Foundation of China(No.21007027);the Qing Lan Project of Jiangsu Province,China;the National"111"Project of China’s Higher Education(No.B12009)

摘　　要：To better understand the role of organic matter(OM)prepared from chicken manure and agriculture residues compost on the growth of plants(Lepidium sativum L.)and antagonistic fungi(Trichoderma harzianum),we analyzed the structure and composition of extracted OM using fluorescence excitation-emission matrix(EEM)spectroscopy and solid-state13C cross-polarization magic-anglespinning nuclear magnetic resonance(13C CPMAS NMR)spectroscopy.The results showed that the EEM contours of water-extracted OM(WEOM)and alkali-extracted OM(AEOM)were similar.Furthermore,solid-state13C CPMAS NMR spectroscopy demonstrated that water extraction could not proportionally pull out aromatic moieties(112–145 ppm)from compost,but the alkali method in proportion extracted both carbohydrates(65–85 ppm)and aromatic moieties.The results suggest that AEOM may better reflect the bulk OM composition of compost,and one should be cautious when applying WEOM as an alternative indicator of total compost OM.Further investigations demonstrated that,compared to carbohydrates,aromatic moieties played a predominant role in growth suppression of Lepidium sativum L.seeds and Trichoderma harzianum.To better understand the role of organic matter （OM） prepared from chicken manure and agriculture residues compost on the growth of plants （Lepidium sativum L.） and antagonistic fungi （Trichoderma harzianum）, we analyzed the structure and composition of extracted OM using fluorescence excitation-emission matrix （EEM） spectroscopy and solid-state 13C cross-polarization magic-angle- spinning nuclear magnetic resonance （13C CPMAS NMR） spectroscopy. The results showed that the EEM contours of water-extracted OM （WEOM） and alkali-extracted OM （AEOM） were similar. Furthermore, solid-state 13C CPMAS NMR spectroscopy demonstrated that water extraction could not proportionally pull out aromatic moieties （112-145 ppm） from compost, but the alkali method in proportion extracted both carbohydrates （65 85 ppm） and aromatic moieties. The results suggest that AEOM may better reflect the bulk OM composition of compost, and one should be cautious when applying WEOM as an alternative indicator of total compost OM. Further investigations demonstrated that, compared to carbohydrates, aromatic moieties played a predominant role in growth suppression of Lepidium sativum L. seeds and Trichoderma harzianum.

关 键 词：excitation-emission matrix spectroscopy extracted organic matter fluorescence reginal integration analysis nuclear magnetic resonance spectroscopy 

分 类 号：S158[农业科学—土壤学] S141.4[农业科学—农业基础科学]