桃品种特异性荧光SSR分子标记数据库构建  

Construction of peach genotype database with Fluorescent-labeled SSR markers

作　　者：李雄伟[1] 孟宪桥[1] 贾惠娟[1] 王力荣[2] 王志强[2] 马瑞娟[3] 吴大军 董冰 Maria Jose Aranzana Pere Arús 高中山[1] 

机构地区：[1]浙江大学果树科学研究所·农业部园艺植物生长发育与生物技术重点开放实验室,杭州310058 [2]中国农业科学院郑州果树研究所,郑州450009 [3]江苏省农业科学院园艺研究所,南京210014 [4]浙江奉化水蜜桃研究所,浙江奉化315521 [5]山西省农业科学研究院果树研究所,山西太谷030815 [6]Centre de Recerca en Agrigenomica CSIC-IRTA-UAB,Campus UAB-Edifici CRAG Bellaterra-Cerdanyola del Valles 08193,Barcelona,Spain

出　　处：《果树学报》2013年第6期924-932,共9页Journal of Fruit Science

基　　金：科技部中国-欧盟科技合作项目(1114);公益性行业(农业)科研专项果树遗传改良与控制技术研究及其应用(200903044);浙江省高校重大科技攻关项目(ZD2009007);浙江省农业新品种选育重大科技专项(2012C12904)

摘　　要：【目的】选择通用的荧光SSR分子标记建立桃种质资源基因型数据库便于进行种质特异性鉴定、资源交换、管理和利用。【方法】根据48个SSR标记对国内外669份桃品系的遗传多样性测试结果，从中选择出一部分多态信息含量较高、适合品种鉴别的标记，采用不同荧光标识组合在ABI3130测序仪测试，为了使不同批次的测试结果准确可靠，6—8个参照品种纳入每次的测试中。结果用16个SSR标记基本可以判断品种的特异性，它们可以用4种荧光标识进行标记，便于分为4组在ABI遗传分析仪上测试。目前数据库包含641份材料．基因型一致的品系多为同一品种突变体和自交系演化的地方品种。文中列出了100份中国代表性种质资源分子标记数据库，并建议8个中国品系为参照。对17个桃品种进行系谱验证发现，我国资源圃所育成的7个品种系谱信息正确，但日本桃品种系谱信息与文献出现偏差。【结论】应用16个多态性高的SSR荧光标记可以构建精确的桃品种分子指纹数据库，测试方法及结果对品种特异性和系谱鉴定提供重要的参考．推动资源分子指纹数据库标准化建设工作。[Objective]The objective of the study was to select a set of tluorescence-labeled SSR markers to construct the genotype database for a large set of peach accessions which will be useful for variety iden tity, management, utilization and exchange of genetic resources. [Method] Based on the polymorphism information content （PIC） of 48 SSR markers in 669 peach accessions, we selected a minimum numbers of SSR that could distinguish most of accessions. These highly polymorphic fluorescent-labeled SSRs were analyzed in groups by ABI system. And 6 to8 reference cultivars were included in each test to make sure the fragment sizes were accurate and comparable. [Result] We selected out 16 SSRs that could be tested by ABI system into four groups. The current fingerprint database comprised of 641 accessions, those with identical genotype are derived from the same cultivar/clonal variation or local varieties from self-crossing.This paper provided the detailed genotype of one hundred Chinese peach collections, including the reference cultivars. The pedigree information of seven groups of Chinese cultivars were confirmed to be con- sistent with records, but for those 8 Japanese cultivars, the pedigree information was deviated from our analysis. [Conclusion] A set of 16 high polymorphic SSR markers can be used to established database of most peach genetic resources. The methodology and data described here provided valuable information for cultivar identification and can be applied for pedigree validation and deduction. It is possible to set up standard molecular marker fingerprint database for the current and future cultivars.

关 键 词：桃 荧光标记SSR 指纹图谱数据库 品种鉴定 系谱鉴定 

分 类 号：S662.1[农业科学—果树学]