甘蓝型油菜幼苗对光氧化胁迫的生理响应  

Response of physiological changes in rapeseed seedling under oxidative stress

作　　者：李俊[1,2] 晁赢[1] 官春云[2] 马霓[1] 张春雷[1] 

机构地区：[1]中国农业科学院油料作物研究所,农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [2]湖南农业大学,湖南长沙410128

出　　处：《中国油料作物学报》2013年第5期551-556,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31101124);公益性行业(农业)科研专项(201203096);现代农业产业技术体系建设专项(CARS-13);国家科技支撑计划课题(2009BAD01B09)

摘　　要：研究油菜光氧化胁迫的生理响应对明确其抗性机理具有重要意义。以甘蓝型油菜中双11号为植物材料,对盆栽幼苗叶片进行光氧化剂甲基紫精(MV)处理,分析测定24h内叶片生理指标的变化。油菜叶片受光氧化胁迫后,抗氧化酶活性、超氧自由基合成速率和丙二醛(MDA)的含量有所提高,并随着处理时间的增加呈现先升高后降低的变化趋势,其中SOD(超氧化物歧化酶)活性在4h达到最大值485.16U·g-1FW,较对照高72.74%;POD(过氧化物酶)活性、CAT(过氧化氢酶)活性、超氧自由基合成速率以及MDA含量均在6h时达到最大值,分别比对照高69.48%,62.81%,98.65%和79.17%;同时,光氧化胁迫降低了可溶性蛋白和可溶性糖含量。回归分析表明短期光氧化胁迫影响油菜高光效的生理因素主要有SOD、POD、CAT和可溶性蛋白含量,其中最重要的效应因子是CAT。A pot experiment was conducted to investigate physiological indexes of rapeseed （Brassica napus L.cv Zhongshuang 11） 0 to 24h after daubing leaves with methyl viologen （MV）.Results indicated that antioxidant enzymes activity,superoxide radical （O2 ＇-） generation rate and MDA content were increased,while Pn and the content of soluble sugar were decreased,during the incipient stage of oxidation stress.Antioxidant enzymes activity,O2.-generation rate,content of MDA and soluble sugar increased at the beginning then decreased.SOD activity reached the peak value of 485.16U · g-1FW at 4h,while activity of POD and CAT,O2.-generation rate and MDA content went to the peaks at 6h,which increased 69.48％,62.81％,98.65％ and 79.17％ respectively compared to CK.Regression analysis indicated that physiologic factors as SOD,POD,CAT,MDA and soluble protein influenced the high photosynthetic rate in rapeseed under short oxidant stress in this experiment,and CAT was the most important factor.

关 键 词：油菜幼苗 光氧化胁迫 甲基紫精 生理响应 

分 类 号：S565.401[农业科学—作物学]