大豆品种耐盐性的快速鉴定法及不同时期耐盐性的研究  

Fast identification method of salt-tolerance and research on salt-tolerance at different stages of soybean cultivars

作　　者：张鹏[1] 徐晨[2] 徐克章[3] 张治安[3] 李大勇[3] 季平[3] 冯引弟[3] 

机构地区：[1]吉林农业大学园艺学院,吉林长春130118 [2]吉林农业大学中药材学院,吉林长春130118 [3]吉林农业大学农学院,吉林长春130118

出　　处：《中国油料作物学报》2013年第5期572-578,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金项目(31171459);国家自然科学基金项目(30871547)

摘　　要：为了探索大豆品种耐盐性的简易鉴定方法,了解不同生育期大豆品种耐盐性的表现,从种子萌发开始用80mmol/L盐溶液对99个大豆品种的耐盐性进行了鉴定和生长观测。结果表明,80mmol/L盐溶液浸泡72h后的大豆发芽率可较好地反映大豆品种的耐盐性。种子萌发后根系的长度、侧根数量、根的干重和鲜重与品种的耐盐性密切相关。苗期大豆对盐胁迫的处理最为敏感,在营养生长期(V2和V4期)盐胁迫显著降低植株的高度和生物量,R2和R4期大豆品种的耐盐性最强,是生物量下降最小的时期。盐胁迫对R6期植株的伤害最大,种子萌发期的耐盐性表现与植株生长后期的耐盐性基本一致,种子萌发期的发芽率可以作为抗盐性的鉴定方法。To search the simple identification method of salt tolerance in soybean cultivars and understand soybean cultivars response to salt tolerance at different stages,80mmol/L salt solution was used to identify salt tolerance in 99 soybean cultivars from seed germination.The results showed that 72h germination percentages with 80mmoL/L salt solution soaking could be used as a method in salt resistance identification and screening.The root length,number of branch root,dry weight and fresh weight of roots were closely correlated with the salt tolerance after seed germination.Soybean cultivars were the most sensitive to salt stress at seeding stage.The salt-sensitive cultivars cannot grow seedlings with 80mmol/L salt solution and this period is also important for understanding salt tolerance of soybean cultivars.At vegetative stage （V2 and V4）,the height and biomass of plant were decreased significantly under salt stress.Salt tolerances were the greatest and biomasses had the least decrease at R2 and R4 stages.Salt stress showed the greatest damage to the plant at R6 stage,namely leaf abscission and premature aging.The salt tolerance of germination period was consistent with that of late growth of plants.Germination percentage of germination period can be used to identify salt tolerance of soybean plants.

关 键 词：大豆 盐胁迫 发芽率 筛选 生物量 

分 类 号：S565.101[农业科学—作物学]