用于大豆根冠关系研究的嫁接方法  

Grafting techniques for root-shoot relationship study in soybean

作　　者：曹晓宁[1,2] 孙石[2] 吴存祥[2] 韩天富[2] 杨文钰[1] 

机构地区：[1]四川农业大学,四川雅安625014 [2]中国农业科学院作物科学研究所,农业部北京大豆生物学重点实验室,北京100081

出　　处：《中国油料作物学报》2013年第5期579-583,共5页Chinese Journal of Oil Crop Sciences

基　　金：农业部财政部现代农业产业技术体系建设专项(CARS-04)

摘　　要：用三个品种（中黄30、中黄39和翼豆12）研究幼苗期不同插接方式对大豆成活率和缓苗天数的影响,以期建立可用于大豆根冠关系研究的嫁接技术.试验采用插接法.对砧木保留两片子叶时接穗去留子叶对成活率的影响,以及接穗保留子叶时,砧木去留子叶对成活率的影响进行研究.结果表明：插接法的成活率可达90％以上;砧木留子叶＋接穗去子叶的嫁接方法缓苗天数需要3～4d;砧木留子叶时,接穗去留子叶对嫁接成活率不会产生显著影响;接穗留子叶时,嫁接部位的不同（即砧木去留子叶）对嫁接成活率没有显著影响;砧木留子叶＋接穗不留子叶的嫁接方式具有一定优势.Effects of different cut-in grafting methods on the survival rate and recover day of grafted soybean plants in soybean young seedlings （Vc-V1） stage were studied to develop a grafting technique for shoot-root relationship research of soybean.Three cultivars were used,Zhonghuang 30 （ZH30）,Zhonghuang 39 （ZH39） and Jidou 12（JD12）,for cut-in grafting method,and two treatments were designed as follows：scion with and without cotyledons were grafted on the stock with cotyledons; scion with cotyledons were grafted on the stock with and without cotyledons,respectively.The results showed that the mean survival rate of cut-in grafting method was above 90％.The recover days of stock with cotyledons and scion without cotyledons treatments were 3-4d.The treatment remained scion cotyledons and stock cotyledons showed a significant impact on the survival rates of the grafted plants.Scion treatments did not show significant impact on the survival rates of stock with cotyledons treatment.The difference of grafting site did not affect the survival rate of grafted plants.But the combination of stock with cotyledons and scion without cotyledons was superior to others.

关 键 词：大豆 嫁接 成活率 根冠关系 

分 类 号：S565.101[农业科学—作物学]