植物根际促生菌辅助红麻修复铅污染土壤  

Phytoremediation of Pb Polluted Soil by Kenaf with Assistance of Plant Growth Promoting Rrhizobectrium(PGPR)

作　　者：陈燕玫[1] 柏珺[1] 杨煜曦[1] 王诗忠[1,2] 杨秀虹[1,2] 仇荣亮[1,2] 

机构地区：[1]中山大学环境科学与工程学院,广州510275 [2]广东省环境污染控制与修复技术重点实验室,广州510275

出　　处：《农业环境科学学报》2013年第11期2159-2167,共9页Journal of Agro-Environment Science

基　　金：国家杰出青年科学基金(41225004);国家"十二五"863重点项目(2012AA06A202);国家自然科学基金(41101483);教育部博士点基金(20110171120028)

摘　　要：通过盆栽实验研究土壤Pb浓度对经济作物红麻(Hibiscus cannabinus)生长、富集及转运Pb的影响,并将具有较强Pb抗性的植物根际促生菌(PGPR)DBM1(Arthrobacter sp.)接种至红麻根际,考察Pb胁迫下PGPR对红麻的促生作用,以探索利用PGPR辅助重金属耐性植物红麻对Pb污染土壤进行植物稳定修复的可行性。结果表明,土壤Pb浓度和接菌处理均显著影响红麻的生长。红麻对Pb具有较高耐性,可通过将Pb富集在根部,并抑制其向地上部转移,从而在中低Pb(Pb400和Pb800处理)污染土壤中良好定植和生长。土壤Pb浓度达到1600 mg·kg-1时,红麻生长开始受到Pb胁迫的抑制,红麻通过自身胁迫抵抗机制缓解Pb毒性。接种DBM1可有效促进红麻的生长,提高红麻叶绿素含量。DBM1对红麻的促生作用是由胁迫诱导的特性,在高Pb胁迫下促生效果更显著。因此,可利用植物根际促生菌DBM1辅助红麻对高Pb污染土壤进行植物稳定修复,在促进红麻生长的同时,有效抑制Pb向红麻地上部的转移。Phytoremediation is a promising option to remediate soils contaminated by heavy metals. Plant growth promoting rhizobectrium （PGPR） may further enhance phytoremediation efficiency. In a pot experiment, the impact of Pb on the growth of and Pb accumulation and translocation in kenaf（Hibiscus cannabinus ） with inoculation of a Pb resistant PGPR DBM1 （Arthrobacter sp. ） was studied. Kenaf had high resistance to Pb and could grow well upto 800 mg Pb· kg-1 soils. The tolerance of kenaf to Pb could result from Pb accumulation in the roots and limited translocation to the shoots （TF〈 1 ）. The growth of kenaf was inhibited at 1600 nag Pb· kg-1 soil. Inoculation of DBM 1 effectively increased biomass and chlorophyll contents of kenaf, with more effective at higher Pb stress. It was concluded that kenaf could be used in combination with PGPR to phytoremediate Pb severely contaminated soils.

关 键 词：红麻 植物根际促生菌 植物稳定 PB 

分 类 号：X563.5[环境科学与工程—环境工程]