转乙肝抗原蛋白基因苜蓿胚胎毒性和致畸性研究  

A Study on Embryotoxicity and Teratogenicity of Alfalfa Transformated with HBV Antigen Protein Gene in Rats

作　　者：夏勇[1] 郑云燕[1] 宋燕华[1] 毛光明[1] 江敏[1] 孟真[1] 

机构地区：[1]浙江省疾病预防控制中心,浙江杭州310051

出　　处：《浙江预防医学》2013年第11期4-7,15,共5页Zhejiang Journal of Preventive Medicine

基　　金：浙江省医药卫生科学研究基金(2009B039)

摘　　要：目的采用动物实验对转乙肝抗原蛋白基因苜蓿（ATHAPG）的大鼠胚胎毒性和致畸性进行研究，为探索ATHAPG对人体健康影响和制订每日允许摄入量提供依据。方法将受孕sD大鼠随机分为40、200和1000mg／kg3个剂量组及阴性对照组（蒸馏水）。每组12～14只孕鼠，于孕期7～16d每天给受试动物灌胃1次，妊娠第20天处死，检查孕鼠妊娠与胎鼠畸形情况。结果3个剂量组的孕鼠生殖能力、体熏及胎鼠体重、外观发育、骨骼和内脏畸形率与阴性对照组比较，差异均无统计学意义（P〉0．05）。结论在该试验条件下，ATHAPG在40～1000mg／kg剂量范围对大鼠无明显的母体毒性、胚胎毒性和致畸性。Objective To assess the embryotoxicity and teratogenicity of alfalfa transformated with HBV antigen protein gene （ATHAPG） in rats and provide evidence for further study of its impact on human health and its acceptable daily intake （ADI）. Methods Pregnant SD rats were randomly divided into five groups with 12 - 14 in each group. ATHAPG was fed at dietary concentrations of 40,200 and 1000 mg/kg groups from day 7 to 16 of gestation. A comparison group received a diet with distilled water. The rats were killed on day 20 and detected the fetal rat malformations. Results There was no significant difference among the three dose groups and the negative controls in the pregnancy rate, the weight of pregnant rats, fetal weight, fetal growth, fetal internal organs and fetal malformation rate. Conclusion There is no maternal toxicity, embryo toxicity and teratogenicity for rats when ATHAPG in the range 40 - 1000 mg/kg.

关 键 词：转乙肝抗原蛋白基因苜蓿 大鼠 胚胎毒性 致畸性 

分 类 号：R965.3[医药卫生—药理学]