肝炎肝硬化患者血常规和骨髓祖细胞造血功能的改变  

CHANGE OF BLOOD ROUTINE AND HEMATOPOIETIC FUNCTION OF BONE MARROW PROGENITOR CELLS IN PATIENTS WITH VIRAL HEPATITIS CIRRHOSIS

作　　者：陆光生[1] 景晔[1] 徐和福[1] 孟震[1] 

机构地区：[1]北京军区天津疗养院,天津300381

出　　处：《解放军预防医学杂志》2013年第5期421-424,共4页Journal of Preventive Medicine of Chinese People's Liberation Army

摘　　要：目的观察肝炎肝硬化患者骨髓祖细胞造血功能和血液常规的变化,为对该类患者采用新的临床治疗措施提供理论依据。方法随机选择26例病毒性肝炎肝硬化患者作为试验组(代偿期、失代偿期肝硬化患者各13例);随机选择13例本院健康体检者作为对照组。对2组观察对象进行骨髓晚期红系祖细胞(CFU-E)、粒-单系祖细胞(CFU-GM)及成纤维祖细胞(CFU-F)体外培养,观察2组间三系的变化及外周血象、血浆白蛋白变化。结果失代偿期肝炎肝硬化患者的血红蛋白、白细胞、血小板白蛋白水平、白球比均明显低于健康对照组和代偿期肝炎肝硬化组的水平(P<0.01);而球蛋白水平则明显高于健康对照组和代偿期肝炎肝硬化组的水平(P<0.01)。代偿期及失代偿期肝炎肝硬化患者CFU-E、CFU-GM、CFU-F集落生长不良率分别为76.9%、30.8%、38.5%及92.3%、69.2%、84.6%,明显高于对照组三系祖细胞集落生长不良率(0%、0%、0%),2组间差异有显著性(P<0.01)。结论与健康对照组比较,肝炎肝硬化患者骨髓祖细胞造血功能明显异常。Objective To observe the change of hematopoietic function of bone marrow progenitor cells and blood routine in patients with viral hepatitis cirrhosis, so as to provide theoretical basis for new therapy for viral hepatitis cirrhosis. Methods Twenty-six patients （ experimental group） with viral hepatitis cirrhosis （ including 13 cases in compensation period, and 13 cases in decompensation period）, and also 13 healthy persons （control group） were randomly selected from our sanatorium. Colony-forming unit-erythroid（ CFU-E）, colony-forming unit- granulocyte and monocyte （ CFU-GM）, colony-forming unit-fibroblast （CFU-F） from bone marrow progenitor cells in both experimental groups and control group were cultured and counted. The level of RBC, WBC, platelets in pe- ripheral blood, and plasma protein was observed too. Results The level of hemoglobin, WBC, platelet, plasma albu- min, and A/G in patients with viral hepatitis cirrhosis in decompensation period was lower （P〈0.01） ,while globu- lin was higher （ P〈0. 01 ） than that in the control and in whom in compensation period. The abnormal rate of colony growth of CFU-E, CFU-GM, CFU-F in both experimental groups during compensation and decompensation period （76.9% ,30.7% ;38.4% ,92.3% ;and 69.2% ,84.6% respectively） was higher than that in control group （0%, 0% ,0% ）, （ P〈0.01 ）. Conclusion The bone marrow progenitor cells in patients with viral hepatitis cirrhosis show obvious abnormal hematopoietic function.

关 键 词：病毒性肝炎肝硬化 血常规 骨髓祖细胞 异常造血 

分 类 号：R373.2[医药卫生—病原生物学]