白细胞介素-27在干燥综合征患者血清的表达及其对白细胞介素-10的调节  

Expression of interleukin 27 in the sera of patients with Sj（o）gren＇s syndrome and its regulation on interleukin 10

作　　者：张璐[1] 任义乐[1] 赵成[1] 李霞[1] 冯学兵[1] 孙凌云[1] 

机构地区：[1]南京医科大学附属鼓楼医院风湿免疫科,210008

出　　处：《中华风湿病学杂志》2013年第11期743-746,F0003,共5页Chinese Journal of Rheumatology

基　　金：南京市青年科技人才启动项目（QYK10152）

摘　　要：目的 探讨白细胞介素（IL）-27在干燥综合征（SS）患者血清表达水平及其对T细胞来源IL-10的调节.方法 采用酶联免疫吸附试验（ELISA）检测34例SS患者与33名健康对照者血清IL-27和IL-10水平.采集SS患者外周血,淋巴细胞分离液分选外周血单个核细胞,分成2组,分别给予植物血凝素（PHA）或PHA＋IL-27刺激,72 h后收集细胞,流式细胞术检测CD3＋CD8-IL-10＋细胞百分率,实时荧光定量聚合酶链反应术检测IL-10转录因子c-Maf mRNA表达.应用配对t检验比较2组细胞百分率及mRNA表达差异,应用t检验比较2组IL-27水平差异,选用Pearson相关分析及多元线性回归分析统计IL-27与IL-10及临床指标的关系.结果 SS患者血清IL-27水平[（158±34） pg/ml]显著高于健康对照者[（139±18） pg/ml,t=2.823,P＜0.01],与IL-10、IgG水平呈正相关（r=0.384,P＜0.05；r=0.354,P＜0.05）,与SS疾病活动度评分（SSDAI）呈负相关（r=-0.503,P＜0.01）,多元线性回归分析显示IL-27与IL-10、SSDAI有线性关系（β=0.412,P＜0.01；β=-0.525,P＜0.01）.PHA＋IL-27刺激组较PHA刺激组CD3＋CD8-IL-10＋细胞百分率增高（P＜0.01,14例）,c-Maf mRNA水平也增高（P＜0.01,22例）.结论 IL-27可促进CD3＋CD8-T细胞表达抑炎性细胞因子IL-10,参与对SS病情调控.Objective To measure the level of interleukin （IL）-27 in sera of patients with Sj（o）gren＇s syndrome （SS） and investigate its role in IL-10 in CD3＋CD8-T cells.Methods Serum levels of 1L-27 and IL-10 from 34 SS patients and 33 healthy controls were tested by enzyme linked immunosorbent assay （ELISA）.Peripheral blood monocytes （PBMCs） from SS patients were collected and cultured in different conditions （one with PHA,the other with PHA and IL-27）.Seventy-two hours later,cells were harvested and the percentages of CD3 ＋CD8-IL-10＋ cells were measured by flow cytometry.Expressions of c-Maf,IL-10 transcription factor,were examined using real-time polymerase chain reaction （PCR）.The data were analyzed by t test,Pearson＇s correlation analysis and multiple linear regression analysis.Results Serum level of IL-27 in patients with SS [（158±34） pg/ml] was significantly higher than that in healthy controls [（139±18） pg/ml,t=2.823,P＜0.01].IL-27 level was positively correlated with IL-10 and IgG levels （r=0.384,P＜0.05; r=0.354,P＜0.05）,but negatively correlated with SSDAI （r=0.503,P＜0.01）.Multiple regression analysis showed independent correlation of IL-27 with IL-10 and SSDAI in SS patients （β=0.412,P＜0.01; β=-0.525,P＜0.01）,but not IgG.CD3＋CD8-IL-10＋ cells was upregulated by IL-27 in vitro （P＜0.01,n=14）,so was c-Maf （P＜0.01,n=22）.Conclusion IL-27 could ameliorate disease activity of Sjogren＇s syndrome.

关 键 词：干燥综合征 T淋巴细胞 白细胞介素10 白细胞介素27 

分 类 号：R593.2[医药卫生—内科学]