实时组织弹性成像弥散定量分析技术评估大鼠肝纤维化的实验研究  

Real-time tissue elastography with tissue dispersion quantitative analysis technique for assessment of rat liver fibrosis

作　　者：徐宇雪[1] 贾春梅[1] 陈武[1] 李淳[1] 郝艳红[1] 陈敏[1] 郭小海[1] 

机构地区：[1]山西医科大学第一医院超声科,太原030001

出　　处：《中华超声影像学杂志》2013年第10期893-896,共4页Chinese Journal of Ultrasonography

摘　　要：目的 探讨实时组织弹性成像（RTE）弥散定量分析技术评估肝纤维化程度的价值.方法 应用6％硫代乙酰胺对51只大鼠制作肝纤维化模型,注射同等量生理盐水的9只大鼠作为对照组.于造模的4周、8周、12周分别从造模组中随机取14只,从对照组中随机取3只行RTE检查,并行组织弥散定量分析,获取12个弹性定量参数,包括应变均值（MEAN）、标准偏差（SD）、蓝色区域％（％AREA）、复杂度（COMP）、峰度（KURT）、偏度（SKEW）、对比度（CONT）、均等性（ENT）、杂乱度（IDM）、一致性（ASM）、相关性（CORR）及肝弹性指数（LF index）.随后处死大鼠,取其肝脏行肝纤维化病理分期,并将造模组分为S0、S1、S2、S3、S4组,将各组12个弹性定量参数进行对照研究.结果 49只大鼠造模成功,纳入分析的有42只.除COMP、KURT、CORR外,余9个弹性定量参数多组间比较差异均有统计学意义（P＜0.05）,且与病理分期均存在相关性,其中MEAN、％AREA及LF index的相关系数较大（r=-0.831、0.882、0.866）.分别以MEAN、％AREA及LF index绘制判断肝纤维化分期的ROC曲线,S≥S1、S≥S2、S≥S3、S=S4时曲线下面积分别为0.884、0.925、0.934、0.962 （MEAN）,0.917、0.958、0.984、0.962（％ AREA）,0.917、0.948、0.966、0.967（LF index）.结论 RTE弥散定量分析技术作为一种无创性检查方法,能够定量评估肝纤维化分期.Objective To explore the value of real-time tissue elastography （RTE） with tissue dispersion quantitative analysis technique for assessment of liver fibrosis stage.Methods 51 rats were injected 6％ thioacetamide to induce liver fibrosis model,and 9 rats were injected saline as control group.In modeling 4 weeks,8 weeks,12 weeks respectively,14 rats in group of liver fibrosis model and 3 rats in control group were randomly selected to RTE.All the rats underwent tissue dispersion quantitative analysis,to obtain 12 quantitative parameters of elasticity,which included average relative strain value （MEAN）,standard deviation of relative strain value （SD）,area ratio of low-strain region （％ AREA）,complexity （COMP）,kurtosis （KURT）,skewness （SKEW）,contrast （CONT）,entropy （ENT）,inverse difference moment （IDM）,angular second moment （ASM）,correlation （CORR） and liver elasticity index （LF index）.Subsequently,rats were sacrificed and their livers were taken for pathology analysis.Liver fibrosis model group was divided into S0,S1,S2,S3,S4 group.The 12 quantitative parameters of elasticity were compared with each group.Results 49 rats were successfully modeled,and 42 rats were analyzed.Except COMP,KURT,CORR,the other quantitative parameters had statistically differences （P ＜ 0.05）.The other 9 parameters were correlated with liver fibrosis stage.Among these parameters,MEAN,％ AREA and LF index had higher related coefficient（r =-0.831,0.882,0.866）.The ROC curve was made by MEAN,LF index and ％AREA to estimate the fibrosis stage,when S≥S1,S≥S2,S≥S3,S =S4,the areas under the ROCcurve were 0.884,0.925,0.934,0.962 （MEAN）;0.917,0.958,0.984,0.962 （％AREA）;0.917,0.948,0.966,0.967 （LF index）,respectively.Conclusions As a non-invasive examination,RTE dispersion quantitative analysis technology can be used to quantitatively assess liver fibrosis.

关 键 词：超声检查 肝硬化 实验性 弹性成像 

分 类 号：R445.1[医药卫生—影像医学与核医学] R575.2[医药卫生—诊断学]