猪圆环病毒2型Rep/Rep'和Cap蛋白亚单位疫苗免疫保护性试验  

Immune protection of porcine circovirus type 2 Rep/Rep' and Cap protein as a subunit vaccines

作　　者：刘丹[1,2] 王一平[1] 吴洪丽[1] 危艳武[1] 刘建波[1] 李胜斌[1] 魏萍 刘长明[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室,黑龙江哈尔滨150001 [2]东北农业大学动物医学院,黑龙江哈尔滨150030

出　　处：《中国预防兽医学报》2013年第11期916-919,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金："863"家畜病毒病基因工程疫苗创制(2011AA10A208);公益性行业(农业)专项(201203039)

摘　　要：猪圆环病毒2型(PCV2)ORF1基因编码的Rep/Rep'蛋白与病毒复制酶相关,ORF2编码的Cap蛋白为病毒核衣壳,是病毒保护性抗原。为比较这3种蛋白作为亚单位疫苗的免疫保护性,本研究以重组杆状病毒表达的PCV2-rRep、-rRep'和-rCap蛋白制备亚单位疫苗及PCV2灭活苗,分组免疫BALB/c鼠进行攻毒,以评价疫苗的免疫保护力。对PCV2-rRep和-rRep'亚单位疫苗免疫鼠血清ELISA抗体检测表明,于免疫后第14 d抗体全部转阳,第35 d抗体效价分别达到1∶42 667和1∶51 200。PCV2-rCap亚单位疫苗和PCV2灭活苗免疫组于第21 d抗体转阳,第35 d抗体效价分别达1∶2 133和1∶333。攻毒试验表明,PCV2-rCap亚单位疫苗和PCV2灭活苗免疫鼠获得完全保护;而PCV2-rRep和-rRep'蛋白亚单位疫苗免疫组无保护作用。血清中和试验表明,PCV2-rCap蛋白亚单位疫苗和PCV2灭活苗免疫鼠血清中和抗体效价达1∶1 600和1∶800;而PCV2-rRep和-rRep'蛋白亚单位疫苗免疫鼠血清无中和病毒能力。该研究表明,PCV2-Cap蛋白亚单位疫苗具有良好的免疫保护力,可以与PCV2自然感染猪产生的抗Rep蛋白抗体相鉴别,具有良好的开发前景。Porcine circovirus type 2 （PCV2） ORF1 encoding Rep/Rep＇ proteins involved in viral replication, and the ORF2 encoding Cap protein consists of viral capsid. To evaluate the immune protection of the three proteins, the baculovirus- expressed PCV2 rRep, rRep＇ and rCap proteins were used to prepare subunit vaccines, respectively. BALB/c mice were vaccinated with the three subunits and PCV2 inactivated vaccine as positive control. The results showed that the antibodies in the rRep and rRep＇ subunit groups appeared positive at 14 days post-immunization （DPI） detected by ELISA, and the titers were 1：42,667 and 1：51,200 at 35 DPI, respectively. While, the antibody titers in the groups of rCap subunit and PCV2-inactivated vaccine were 1：2,133 and 1：333 at 35 DPI, respectively. Otherwise, the neutralizing antibody （NA） titers in the sera of rCap subunit and PCV2-inactivated vaccines were 1：1,600 and 1：800 respectively and the protective rate was 100% in the two groups post- challenge. However, the NAs were not detected in rRep and rRep＇ subunit groups and no protection was found in the two groups. In conclusion, the rCap is a promising candidate subunit vaccine against PCV2.

关 键 词：猪圆环病毒2型 REP Rep’和Cap蛋白 亚单位疫苗 免疫保护性 

分 类 号：S852.65[农业科学—基础兽医学]