电穿孔技术对DNA疫苗pVAX-tG250FcGB免疫效果的影响  

Influence of electroporation on immunogenicity of the DNA vaccine pVAX-tG250FcGB

作　　者：肖毅[1,2] 高昆[2] 杨勇[1] 阎瑾琦[2] 张亮[2] 王宇[2] 徐元基[2] 田仁礼[2] 杜芝燕[2] 于继云[2] 

机构地区：[1]中国人民解放军总医院第一附属医院泌尿外科,北京100048 [2]军事医学科学院基础医学研究所,北京100850

出　　处：《南方医科大学学报》2013年第11期1628-1631,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30840094)~~

摘　　要：目的探讨应用电穿孔技术对DNA疫苗pVAX-tG250FcGB免疫效果的影响。方法构建肾癌DNA疫苗pVAXtG250FcGB,通过体内电穿孔技术或普通肌肉注射途径,将DNA疫苗导入到BALB/c小鼠体内,探讨电穿孔免疫途径诱导抗原特异性免疫应答的效果。结果电穿孔技术或普通肌肉注射均能在小鼠体内诱导出抗原特异性的体液免疫应答和细胞免疫应答,电穿孔技术的免疫效果明显优于普通肌肉注射途径。结论电穿孔技术介导的DNA疫苗pVAX-tG250FcGB免疫具有较强的诱导免疫应答的能力,是研究DNA疫苗pVAX-tG250FcGB免疫效果的一种有效途径。Objective To investigate the influence of electroporation on the immtmogenicity of the DNA vaccine pVAX- tG250FcGB. Methods The DNA vaccine pVAX-tG250FcGB was constructed by inserting the coding gene of tG250 fusion genes into the expression vector pVAX. The DNA vaccine was delivered in BALB/c mouse by electroporation or intramuscular injection, and the induced antigen specific immune responses were compared. Results The vaccine delivered by electroporation and intramuscular injection both induced immune responses in BALB/c mouse, but electroporation produced an obviously stronger effect than intramuscular injection. Conclusion Electroporation-mediated DNA vaccine delivery can produce strong immune response in mice and is an effective means for studying the immunogenic effect of DNA vaccine pVAX-tG250FcGB.

关 键 词：肾肿瘤 DNA疫苗 电穿孔 免疫效果 

分 类 号：R392.1[医药卫生—免疫学]