大鼠股骨骨折对肾皮质浅层细胞凋亡的影响及三七总皂苷的保护作用  

Protective effect of panax notoginseng saponins against femoral fracture- induced apoptosis in the superficial cells of rat renal cortex

作　　者：高安敏[1] 冯颖[1] 曾峰[1] 王小梅[1] 王芳[1] 杨兰生[1] 孙少华[1] 

机构地区：[1]兰州大学第一医院病理研究所,甘肃兰州730000

出　　处：《南方医科大学学报》2013年第11期1692-1695,共4页Journal of Southern Medical University

基　　金：甘肃省卫生行业科研计划(GSWST2011-19)

摘　　要：目的探讨大鼠股骨骨折对肾皮质浅层细胞凋亡的影响和三七总皂苷（PNs）对骨折后肾皮质浅层的保护作用。方法将90只Wistar大鼠随机分为单纯骨折组（36只）、骨折给药组（36只）、对照组（18只），前两组又平均分为6组分别在骨折1、6、12、24、36、48h时各处死6只，而对照组在各时间段分别处死3只。采用HE染色观察病理变化及TUNEL染色观察凋亡分布，免疫组化及原位杂交技术检测Bcl-2和Bax在肾皮质中的表达。结果单纯骨折组中肾皮质浅层肾毛细血管扩张，近曲小管颗粒样变性，而骨折给药组与单纯骨折组比较肾损伤较轻。单纯骨折组中，Bcl-2及Bcl-2mRNA表达在1～12h较正常对照组高（P〈0．01）；Bax表达在12～36h高于正常对照组（P〈0．01），BaxmRNA表达24-48h阳性表达高于正常对照组（P〈0．01）。在骨折给药组，Bcl-2表达在1h明显高于单纯骨折组（P〈0．01），Bcl．2mRNA表达在12～24h高于单纯骨折组（P〈0．01）；Bax阳性表达及BaxmRNA表达在24-48h低于单纯骨折组（P〈0．01）。结论骨折对肾皮质浅层Bcl．2和Bax蛋白的表达及mRNA的转录均有明显影响，PNS通过上调抑凋亡基因Bcl-2和下调促凋亡基因Bax表达而抑制细胞凋亡的发生。Objective To study the protective effect of panax notoginseng saponins （PNS） against apoptosis of the superficial ceils of rat renal cortex following femoral fracture in rats. Methods Ninety Wistar rats were randomized into 3 groups, namely the fracture group （n=36）, fracture with PNS treatment group （n=36）, and normal control group （n=18）. At 1, 6, 12, 24, 36, and 48 h after femoral fracture, 6 rats from first two groups and 3 from the control group were sacrificed to observe renal pathologies with HE-staining. Immunohistochemistry and in situ hybridization were used to detect Bcl-2 and Bax expression, and TUNEL staining was employed to detect the distribution of apoptotic cells. Results In the fracture group, the renal cortex showed telangiectasia and granular degeneration of proximal tubule, which were lessened in the PNS treatment group. Compared with the control group, the fracture group showed significantly increased Bcl-2 and Bcl-2 mRNA expressions in the renal cortex at 1-12 h （P〈0~01） and increased Bax protein expression at 12-36 h （P〈0.01） with increased Bax mRNA expression at 24-48 h （P〈0.01）. In PNS treatment group, Bcl-2 protein expression at 1 h and Bcl-2 mRNA expression at 12-48 h were significantly higher （P〈0.01）, but Bax protein and mRNA expressions at 24-48 h were significantly lower （P〈0.01） than those in the fracture group. Conclusion Femoral fracture obviously affects Bcl-2 and Bax protein and mRNA expressions in the superficial cells of the renal cortex, PNS can suppress the cell apoptosis by down-regulating Bax expression and up-regulating Bcl-2 expression.

关 键 词：三七总皂苷 骨折 肾脏皮质 凋亡 

分 类 号：R285.5[医药卫生—中药学]