黑杨三倍体与二倍体叶片中miRNA表达差异研究  

Study on the Difference in Expression Profiles of microRNA Among Leaf Organs of Triploid and Diploid Populus x euramericana cl. ＇ Zhonglin46＇

作　　者：王楠[1] 胡宝全[1] 王春国[1] 宋文芹[1] 陈成彬[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《林业科学研究》2013年第F10期33-38,共6页Forest Research

基　　金：国家“973”计划项目（2009CB119105）,国家自然科学基金重点项目（31371249）

摘　　要：本文以改进CTAB法提取的总RNA为模板，通过设计茎环反转录引物，利用定量RT—PCR技术从黑杨幼嫩叶片、成熟叶片中均检测到miRNA。结果表明，miRl59、miRl67、miRl71、miR319在三倍体幼叶中表达量均高于二倍体，miRl64在三倍体成熟叶片中的表达量高于二倍体表达量。除miR319外，其余miRNA与其相应的靶基因在二倍体和三倍体黑杨不同阶段叶片中表达的消长关系都较为典型。暗示这些miRNA靶基因可能与黑杨三倍体的速生性状形成相关。Using total RNA extracted by CTAB method as templates, and mature leaf organs of Populus x euramericana cl. ＇ Zhonglin46 the expression profiles of 5 miRNAs in young were studied by real-time stem-loop RT-PCR. The results showed that the expression of miR159, miR167, miR171, and miR319 in triploid young leaves were higher than those in diploid, however, the expression of miR164 in mature leaves of triploid was higher than that in diploid. In addition, the expression analysis of the corresponding miRNA target genes showed that the expression of the miRNAs and their corresponding targets were negative correlation in diploid and triploid P. x euramericana cl. ＇ Zhonglin46＇ these findings suggested that these miRNA target genes may be related to the formation of fast-grow- ing traits showed by triploid Populus.

关 键 词：黑杨 叶片组织 MICRORNA 荧光定量PCR 差异表达 

分 类 号：S718.46[农业科学—林学]