黑杨三倍体nSZ丹基因的克隆与功能分析  

Molecular Cloning and Function Analysis of PtSIP3 Gene from Populus × euramericana cl. ＇ Zhonglin 46＇ Triploid

作　　者：胡宝全[1] 韦韬[1] 王春国[1] 宋文芹[1] 陈成彬[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《林业科学研究》2013年第F10期39-44,共6页Forest Research

基　　金：国家“973”计划项目（2009CB119105）

摘　　要：本研究以黑杨中林46三倍体为材料，依据前期表达谱芯片的分析结果，分离克隆得到三倍体材料中高表达基因粥鹏，该基因的编码产物为钙调磷酸酶B（CalcineurinB．1ikeProteins，简称CBL）的靶蛋白。随后构建了双元表达载体pRI101-PtSIP3，并成功的转入拟南芥中。经qRT—PCR表达分析和表型观察，发现PtSIP3在拟南芥中异源过表达会抑制胚和果荚的发育、促进根的快速伸长。推测该基因在黑杨三倍体中的表达升高，可能会参与黑杨三倍体的营养生长期的调控并促进根的发育。According to the preliminary results of microarray analysis, a high expression gene, PtSIP3, was cloned from Populus euramericana triploid, which generated from crosses between the maternal parent P. deltoides and the pollen parent P. nigra. The binary expression vector, pRIIO1-PtSIP3, was constructed and transformed into Arabi- dopsis. So far, the PtSIP3 transgenic plants were observed the remarkable phenotypic changes with inhibition of em- bryo and capsule development, improvement root development. Therefore, It is speculated that the rises of PtSIP3 expression may participate in the regulation of vegetative growth and promote root development in P. triploid.

关 键 词：黑杨 三倍体 PtSIP3 基因表达 

分 类 号：S718.46[农业科学—林学]