杂种落叶松嫩枝离体培养与芽增殖研究  

Research on the Shoot Growth and Bud Propagation In Vitro Culture

作　　者：李魁鹏[1] 王亚珍 孙晓梅[1] 韩华[1] 谢允慧[1] 张守攻[1] 

机构地区：[1]林木遗传育种国家重点实验室,中国林业科学研究院林业研究所,北京100091 [2]青岛市崂山林场,山东青岛266061

出　　处：《林业科学研究》2013年第F10期82-86,共5页Forest Research

基　　金：国家“973”计划项目（2009CB119107）

摘　　要：以生长和生根兼优的杂种落叶松无性系日永8×长混18．10的嫩枝和休眠芽为试验材料，开展了嫩枝生长和芽增殖的离体组织培养技术研究，确定了两种试验材料的最佳灭菌条件及最适培养基。结果表明：15％的次氯酸钠溶液处理10min，材料无菌存活率达到91．7％，灭菌效果最好；最适嫩枝生长培养基为B培养基；芽增殖过程中，最适腋芽萌发诱导培养基为BEMB培养基，单个嫩枝平均诱导萌发2个腋芽；无外源激素的改良BEMB培养基（硝态氮与铵态氮含量比为6：1），最适于腋芽成枝诱导，成枝率为36．5％；培养基MCM＋1．5mg·L-1 ZT＋0．15mg·L-1 KT，最适用于休眠芽不定芽诱导，不定芽诱导率为39．1％，增殖倍数为2．6。Taking the shoot and the dormant bud from fine clone plants of hybrid larch （ Larix kaempferi Riyong 8 x L. olgertsis 18-10） , which is possessed of good variety for both growth and rooting, the tissue culture experiment of shoot growth and bud propagation was conducted. The effect of 15% sodium hypochlorite for 10 mins on sterilization treatment results the best, with a high rate of aseptic survival up to 91.7%. Medium B is suitable for elongation growth of the explant. While the medium BEMB is suitable for axillary buds induction and two axillary buds sprout on one shoot. Among the treatment of induction axillary buds to become branches, the BEMB, of which the content rate of nitrate nitrogen/ammonium nitrogen is 6： 1, without exogenous chemical growth regulators, has the best in- duction effects and the rate of branches formation is 36.5%. Dormant buds for adventitious buds induction was planted on MCM medium with different cytokinin treatment, and the results showed MCM ＋ 1.5 mg L-1 ZT ＋ 0. 15 mg L-1 KT is the best medium for adventitious buds induction. The rate of buds induction is 39.1% and the average buds number induced by a bud is 2.6.

关 键 词：杂种落叶松 嫩枝生长 芽增殖 组织培养 

分 类 号：S722.3[农业科学—林木遗传育种]