ω-3不饱和脂肪酸和谷氨酰胺抗压力性氧化损伤的实验研究  

Effect of ω-3 Unsaturated Fatty Acids and Glutamine Against Resistance Oxidative Damage

作　　者：王艳[1] 高金华[1] 周守凤[1] 陈慧敏[1] 郑国荣[1] 刁波[2] 

机构地区：[1]广州军区武汉总医院消化内科,湖北武汉430070 [2]广州军区武汉总医院神经外科研究所医学实验中心

出　　处：《解放军护理杂志》2013年第20期27-29,36,共4页Nursing Journal of Chinese People's Liberation Army

基　　金：湖北省自然科学基金课题(2012FFB06806);湖北省卫生厅2013-2014年度科研项目(HL2012-8)

摘　　要：目的研究ω-3不饱和脂肪酸(polyunsaturated fattyacids,ω-3PUFAs)和谷氨酰胺(glutalnin,Gln)对老年大鼠受压组织细胞的抗氧化损伤作用,并探讨其可能的机制。方法将SPF级雄性老年SD大鼠28只按随机数字表法分成对照组、模型组、ω-3PUFAs干预组(A组)、Gln干预组(B组)。A、B组大鼠分别给予PUFAs及Gln配制液灌胃喂养20d;对照组不受压,模型组及A、B组早期压疮模型诱导成功后,4组大鼠一起实施安乐死。观察受压组织病理形态学变化;检测各组乳酸脱氢酶(lactate dehydrogenase,LDH)漏出液、10%肌肉匀浆液中超氧化物歧化酶(superoxide dismutase,SOD)的活性及一氧化氮(nitricoxide,NO)含量的变化。结果受压组织病理形态学显示,与模型组相比,A组受压组织受损程度明显减轻(P<0.01),B组受压组织受损程度有所好转(P<0.05)。生化检测显示,A组受压组织SOD活性显著增高、LDH漏出液和NO含量显著减少(均P<0.01);B组受压组织SOD活性增高、LDH漏出液减少(均P<0.05),NO含量显著降低(P<0.01)。结论ω-3PUFAs和Gln早期干预均能提高受压组织抗氧化能力,尤其是ω-3PUFAs对老年大鼠抗压力性氧化损伤作用较强。Objective To study the effect against oxidative damage of ω-3 unsaturated fatty acid （ω-3PUFAs） and glutamine （Gln） on compression tissue cell of elderly rats,and explore its possible mechanisms. Methods Twenty eight SPF-level elderly male SD rats were randomly assigned to four groups, which were the control group, the model group, the ω-3PUFAs intervention group （group A） and the Gln intervention group （group B）, respectively. The rats in group A were fed by lavaging on the ω-3PUFAs configuration liquid and rats in group B were fed by lavaging on the Gln configuration liquid. All of the rats in the four groups were euthanized after the model success- fully built, where the control group were given no pressure while the model group, group A and the group B were induced early bedsore model. Based on that, we observed the pathological morphology change of the pressure tissue,tested the content change of the LDH leakage liquid and 10% muscle homogenate super Oxide dismutase （SOD） activity and nitric oxide （NO）. Results Compared with the model group, the level of the compression tissue in the group A and group B were highly significantly reduced （P〈0.01,P〈0.05）. Biochemical test showed that SOD activity of compression tissue in group A was significantly increased while LDH leakage liquid and NO content was significantly reduced （P〈0.01） ; SOD activity of compression tissue in group B increased while LDH leakage liquid reduced （P〈0. 05） and NO content significantly reduced （P〈0.01）. Conclusion Both the ω-3PUFAs and Gln early intervention can improve compression tissue antioxidant ability, especially the ω-3PUFAs,whose function of compressive resistance in aged rats was strong.

关 键 词：压疮 老年 氧化损伤 Ω-3不饱和脂肪酸 谷氨酰胺 

分 类 号：R197.323[医药卫生—卫生事业管理]