中国北方地区隐源性肝炎及乙型肝炎表面抗原阴性肝癌患者中隐匿性HBV感染流行状况分析  

Prevalence of occult HBV infection among cryptogenic chronic liver disease patients and HBsAg nega-tive HCC patients in north of China

作　　者：房勇[1] 梁爽[1] 陈思佳[1] 徐维祯[1] 赵颖新[2] 谷鸿喜[1] 

机构地区：[1]哈尔滨医科大学微生物学教研室,黑龙江省感染与免疫重点实验室,黑龙江省感染与免疫科技创新团队,150081 [2]哈尔滨医科大学附属第一医院,150001

出　　处：《国际免疫学杂志》2013年第6期472-475,共4页International Journal of Immunology

基　　金：黑龙江省教育厅科技研究项目

摘　　要：目的调查中国北方地区隐源性肝炎及乙肝表面抗原（HBsAg）阴性肝癌患者中隐匿性乙型肝炎病毒（HBV）感染的流行状况。方法收集393个受试者的血清，其中包括隐源性慢性肝炎患者215名、HBsAg阴性肝癌患者178名。使用巢式PCR的方法检测血清中的HBV-DNA，同时使用实时定量PCR的方法检测HBV—DNA的载量。结果在隐源性慢性肝炎患者、HBsAg阴性肝癌患者中隐匿性HBV感染流行率分别为23．7％（51／215）和68．5％（122／178）。在IgGanti—HBc阳性者中，隐匿性HBV感染率较高。所有隐匿性感染者的病毒载量均较低（〈10^5拷贝／ml）。结论在中国北方隐源性肝炎及肝癌患者中，隐匿性HBV感染率较高。因此，对隐匿性HBV感染应给及足够的重视，避免因输血及器官移植造成HBV的传播。Objective To investigate the prevalence of occult HBV infection among cryptogenic chron- ic liver disease patients and hepatitis B surface antigen （HBsAg） negative hepatocellular carcinoma （HCC） pa-tients in north of China. Methods The HBV DNA in 393 sera samples collected from cryptogenic chronic liver disease patients （215）, HBsAg negative hepatocellular carcinoma （HCC） patients （ 178 ） were tested by nested PCR. We performed real-time PCR to determine the levels of serum HBV-DNA. Results Prevalence of occult HBV infection was 23.7% （51/215） and 68.5% （122/178） in cryptogenic chronic liver disease patients, HBsAg-negative HCC patients, respeetivly. The prevalence of occult HBV infection in IgG anti-HBe-positive subjects was significantly high. In all cases ,viral loads were low （ 〈 10^5 viral copies/ml）. Conclusion The prev- alence of occult HBV infection was significantly high among cryptogenic chronic liver disease patients and HB-sAg-negative HCC patients in north of China. Thus, more meticulous attention should be given to prevent HBV transmission by blood transfusion or organ transplantation in endemic areas, and further studies on clinical impli-cation and mechanism of occult HBV infection are required.

关 键 词：乙型肝炎病毒 隐匿性感染 流行率 

分 类 号：R512.62[医药卫生—内科学]