茵陈四苓颗粒乙醇提取工艺优选  

Optimization of Ethanol Extraction Process for Yinchen Siling Granules

作　　者：赵剑[1] 蒲清荣[1] 陈玉兰[1] 黄锐[1] 罗群[1] 

机构地区：[1]泸州医学院附属中医医院,四川泸州646000

出　　处：《中国实验方剂学杂志》2013年第22期31-34,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省泸州医学院课题(2011QN-91)

摘　　要：目的:优选茵陈四苓颗粒的乙醇提取工艺。方法:以栀子苷提取量为指标,通过单因素试验比较乙醇回流法、乙醇超声法和乙醇渗漉法,确定茵陈四苓颗粒中部分药材的提取方法;通过正交试验考察乙醇体积分数、浸泡时间、加醇量、渗漉流速对提取工艺的影响。采用HPLC测定栀子苷含量,色谱条件为UltimateTMC18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-水(15∶85),流速1 mL·min-1,柱温40℃,检测波长238 nm,进样量10μL。结果:采用乙醇渗漉法提取,最佳提取工艺条件为加10倍量70%乙醇浸泡12 h,渗漉流速5 mL·min-1,栀子苷提取量达42.524 4 mg·g-1。结论:该工艺客观可行、稳定合理,可为茵陈四苓颗粒的工业生产提供理论依据。Objective： To optimize ethanol extraction technology of Yinchen Siling granules. Method： With yield of geniposide as index, single factor test was adopted to screen ethanol extraction method, such as refluxing method, ultrasonic method and percolation method; Effects of ethanol concentration, soaking time, ethanol amount and percolation velocity on extraction technology was investigated by orthogonal design. The content of geniposide was determined by HPLC, chromatographic conditions were as follows： UltimateTMCls column （4.6 mm x 250 mm, 5 ~m）, mobile phase of acetonitrile-water （15： 85）, flow rate 1 mL- min-l, column temperature 40 ℃ , detection wavelength 238 nm, injection volume 10 μL. Result： Ethanol percolation method was adopted, the best extraction conditions were as follows： soaked 12 h by adding 10 times the amount of 70% ethanol with percolation velocity of 5 mL min-l; Under these conditions, yield of geniposide was up to 42. 524 4 mg -g-1 Conclusion： This optimized extraction process was stable, reasonable and practical, it could provide a theoretical basis for industrial production of Yinchen Siling granules.

关 键 词：渗漉提取法 正交设计 高效液相色谱 栀子苷 茵陈四苓颗粒 栀子 泽泻 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]