超声辅助碱溶酸沉法提取铁苋菜总黄酮的工艺优选  

Optimization of Ultrasonic-Assisted Alkali Extraction and Acid Precipitation Technology for Total Flavonoids from Acalypha australis

作　　者：崔明筠[1] 徐健蓉[1] 徐秀霞[1] 向发椿[1] 刘俊[2] 

机构地区：[1]西南科技大学,四川绵阳621010 [2]四川省冶金地质勘查局,成都611700

出　　处：《中国实验方剂学杂志》2013年第22期38-40,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：西南科技大学博士基金项目(12zx7108)

摘　　要：目的:优选超声辅助碱溶酸沉法提取铁苋菜总黄酮的工艺条件。方法:以芦丁为对照成分,采用紫外-可见分光光度法测定铁苋菜总黄酮含量。以总黄酮得率为指标,在单因素试验基础上,通过正交试验考察料液比、碱性提取液pH、温度、酸沉pH和时间对铁苋菜总黄酮提取工艺的影响。结果:铁苋菜总黄酮最佳提取工艺为料液比1∶20,碱提液pH 11,温度60℃,酸沉pH 2.5,超声时间90 min,提取数1次;铁苋菜总黄酮提取量24.11 mg·g-1,提取率达87.76%。结论:优选的提取工艺稳定可行、提取率高,为铁苋菜资源的开发利用提供实验依据。Objective： To optimize ultrasonic-assisted alkali extraction and acid precipitation technology of total flavonoids from Acalypha australis. Method： UV spectrophotometry was used to determine the content of total flavonoids with rutin as a control component. With yield of total flavonoids as index, based on single factor test, effects of solid-liquid ratio, pH of alkaline extraction solvent, temperature, acid precipitation pH and time on extraction technology were investigated by orthogonal test. Result： The best extraction technology was as following： solid-liquid ratio of 1 ： 20, pH of extraction solvent 11, extracting temperature of 60 ~C , acid precipitation pH of 2.5, and ultrasonic time of 90 min. Under these conditions, extraction amount of total flavonoids from A. australis was 24.11 mg .g-1, and its yield could reach 87.76% . Conclusion： This optimized technology was stable and feasible with high extraction rate, it could provide experimental basis for development and utilization of A. australis.

关 键 词：铁苋菜 总黄酮 单因素试验 正交试验 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]