HPLC同时测定四磨汤口服液中辛弗林、槟榔碱和去甲异波尔定的含量  

Determination of Synephrine,Norisoboldine and Arecoline in Simo Tang Oral Liquid by HPLC

作　　者：杨华 易小兰 谢金华 张飞飞 郭元满 胡高云[2] 

机构地区：[1]湖南汉森医药研究有限公司,长沙410013 [2]中南大学,长沙410013

出　　处：《中国实验方剂学杂志》2013年第22期79-81,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划项目(2009CB523002);国家"十二五"重大新药创制专项项目(2011ZX09201-201-08)

摘　　要：目的：建立同时测定四磨汤口服液中辛弗林、槟榔碱和去甲异波尔定含量的方法.方法：采用高效液相色谱法,色谱柱Agilent SCX（4.6 mm×250 mm,5μm）,流动相甲醇-0.2％磷酸（2～1 000 mL水,氨水调pH 3.8）（65∶ 35）,检测波长215 nm,流速1 mL· min-1,柱温35℃.结果：辛弗林在0.05～2.55 μg线性良好（r=0.999 9）,槟榔碱在0.13 ～2.59 μg线性良好（r =0.999 8）,去甲异波尔定在0.04～2.01 μg线性良好（r=0.999 9）.辛弗林、槟榔碱和去甲异波尔定平均加样回收率分别为99.8％,100.6％,100.61％.结论：该方法简便、准确、可靠,对四磨汤口服液质量标准提高具有一定参考价值.Objective： To establish a determination method of synephrine, norisoboldine and arecoline in Simo Tang oral liquid by HPLC. Method： HPLC method was set up, column was Agilent SCX, the mobile phase was methanol-0.2% phosphate solution, with the flow rate of 1.0 mL ＂min-1 ; the detection wavelength was set at 215 nm, column temperature was kept at 35 ~C. Result： Synephrine was linear within the range of 0.05-2.55 μg （ r =0. 999 9） ; the average recovery was 99.8%. Norisoboldine was linear within the range of 0.04-2.01 μg （r = 0. 999 9 ） ; the average recovery was 100. 61%. Arecoline was linear within the range of 0. 13-2. 59 μg （r = 0. 999 8） ; the average recovery was 100.6%. Conclusion： The method is simple, accurate and stable, which is valuable to improve the quality standard of Simo Tang oral liquid.

关 键 词：高效液相色谱 四磨汤口服液 辛弗林 去甲异波尔定 槟榔碱 

分 类 号：R284.1[医药卫生—中药学]