HPLC测定家蚕中1-脱氧野尻霉素的含量  

Content Determination of Deoxynojirimycin in Silkworm Powder by RP-HPLC

作　　者：赵艳丽[1] 黄亦琦[1] 胡珊梅[1] 杨辉[1] 

机构地区：[1]厦门市医药研究所厦门市天然药物研究与开发重点实验室,福建厦门361008

出　　处：《中国实验方剂学杂志》2013年第22期124-126,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：福建省自然科学基金项目(2011D006);厦门市科技计划项目(20124040)

摘　　要：目的：建立RP-HPLC测定家蚕中1-脱氧野尻霉素（DNJ）的含量.方法：采用Hypersil NH2色谱柱（4.6 mm×250mm,5μm）,以乙腈-水（78∶22）为流动相,流速1.0 mL·min-1,柱温30℃,检测波长203 nm.结果：家蚕中DNJ在1.996 ～9.98μg（r=0.999 6）峰面积与进样量呈良好的线性关系,平均加样回收率为98.4％.结论：该方法前处理简单、实用,结果准确,可用于家蚕及制品中DNJ的质量控制.Objective：To establish assaying methods for the determination of deoxynojirimycin in silkworm.Method：The content of DNJ was determined by RP-HPLC,using a Hypersil NH2 （4.6 mm × 250mm,5 μm）.The column temperature was maintained at 30 ℃.Acetonitrile-water （78：22） was used as mobile phase; the flow-rate was 1.0 mL ·min-1.The detecting wavelength was set at 203 nm.Result：The linear range was 1.996-9.98 μg （r =0.999 6）.The average recovery was 98.4%.Conclusion：This method is simple,practical and accurate.It could be used for quality control of silkworm.

关 键 词：家蚕 1-脱氧野尻霉素(DNJ) 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]